論文 - 詳細
| RRC ID | 18560 |
|---|---|
| 著者 | Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H. |
| タイトル | Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft. |
| ジャーナル | Cryobiology |
| Abstract |
Insufficient bone union is the occasional complication of biomechanical reconstruction after malignant bone tumor resection using temperature treated tumor bearing bone; freezing, pasteurization, and autoclaving. Since bone morphogenetic protein (BMP) plays an important role in bone formation, we assessed the amount and activity of BMP preserved after several temperature treatments, including -196 and -73°C for 20 min, 60 and 100°C for 30 min, 60°C for 10h following -80°C for 12h as an allograft model, and 4°C as the control. The material extracted from the human femoral bone was treated, and the amount of BMP-7 was analyzed using an enzyme-linked immunosorbent assay. Then, the activity of recombinant human BMP-7 after the treatment was assessed using a bioassay with NIH3T3 cells and immunoblotting analysis to measure the amount of phospho-Smad, one of the signaling substrates that reflect the intracellular reaction of BMPs. Both experiments revealed that BMP-7 was significantly better preserved in the hypothermia groups. The percentages of the amount of BMP-7 in which the control group was set at 100% were 114%, 108%, 70%, 49%, and 53% in the -196, -73, 60, 100°C, and the allograft-model group, respectively. The percentages of the amount of phospho-Smad were 89%, 87%, 24%, 4.9%, and 14% in the -196, -73, 60, 100°C, and the allograft-model group, respectively. These results suggested that freezing possibly preserves osteoinductive ability than hyperthermia treatment. |
| 巻・号 | 63(3) |
| ページ | 235-9 |
| 公開日 | 2011-12-1 |
| DOI | 10.1016/j.cryobiol.2011.09.001 |
| PII | S0011-2240(11)00150-7 |
| PMID | 21963423 |
| MeSH | Animals Biomarkers / analysis Bone Morphogenetic Protein 7 / analysis Bone Morphogenetic Protein 7 / biosynthesis* Bone Morphogenetic Protein 7 / pharmacology Bone Transplantation* Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Femur Head / metabolism* Fibroblasts / drug effects Fibroblasts / metabolism Freezing Hot Temperature Humans Male Mice NIH 3T3 Cells Pasteurization / methods* Phosphorylation / drug effects Recombinant Proteins / analysis Recombinant Proteins / biosynthesis* Recombinant Proteins / pharmacology Signal Transduction / drug effects Signal Transduction / physiology* Smad1 Protein / metabolism* Transplantation, Autologous Transplantation, Homologous |
| IF | 2.287 |
| 引用数 | 35 |
| WOS 分野 | BIOLOGY PHYSIOLOGY |
| オルトメトリクス指標 |
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH3T3/14-1(RCB0056) |