RRC ID 18560
著者 Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y, Kimura H, Miwa S, Takeuchi A, Tsuchiya H.
タイトル Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft.
ジャーナル Cryobiology
Abstract Insufficient bone union is the occasional complication of biomechanical reconstruction after malignant bone tumor resection using temperature treated tumor bearing bone; freezing, pasteurization, and autoclaving. Since bone morphogenetic protein (BMP) plays an important role in bone formation, we assessed the amount and activity of BMP preserved after several temperature treatments, including -196 and -73°C for 20 min, 60 and 100°C for 30 min, 60°C for 10h following -80°C for 12h as an allograft model, and 4°C as the control. The material extracted from the human femoral bone was treated, and the amount of BMP-7 was analyzed using an enzyme-linked immunosorbent assay. Then, the activity of recombinant human BMP-7 after the treatment was assessed using a bioassay with NIH3T3 cells and immunoblotting analysis to measure the amount of phospho-Smad, one of the signaling substrates that reflect the intracellular reaction of BMPs. Both experiments revealed that BMP-7 was significantly better preserved in the hypothermia groups. The percentages of the amount of BMP-7 in which the control group was set at 100% were 114%, 108%, 70%, 49%, and 53% in the -196, -73, 60, 100°C, and the allograft-model group, respectively. The percentages of the amount of phospho-Smad were 89%, 87%, 24%, 4.9%, and 14% in the -196, -73, 60, 100°C, and the allograft-model group, respectively. These results suggested that freezing possibly preserves osteoinductive ability than hyperthermia treatment.
巻・号 63(3)
ページ 235-9
公開日 2011-12-1
DOI 10.1016/j.cryobiol.2011.09.001
PII S0011-2240(11)00150-7
PMID 21963423
MeSH Animals Biomarkers / analysis Bone Morphogenetic Protein 7 / analysis Bone Morphogenetic Protein 7 / biosynthesis* Bone Morphogenetic Protein 7 / pharmacology Bone Transplantation* Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Femur Head / metabolism* Fibroblasts / drug effects Fibroblasts / metabolism Freezing Hot Temperature Humans Male Mice NIH 3T3 Cells Pasteurization / methods* Phosphorylation / drug effects Recombinant Proteins / analysis Recombinant Proteins / biosynthesis* Recombinant Proteins / pharmacology Signal Transduction / drug effects Signal Transduction / physiology* Smad1 Protein / metabolism* Transplantation, Autologous Transplantation, Homologous
IF 2.287
引用数 35
WOS 分野 BIOLOGY PHYSIOLOGY
リソース情報
ヒト・動物細胞 NIH3T3/14-1(RCB0056)