| RRC ID |
18574
|
| Author |
Otsu K, Kishigami R, Oikawa-Sasaki A, Fukumoto S, Yamada A, Fujiwara N, Ishizeki K, Harada H.
|
| Title |
Differentiation of induced pluripotent stem cells into dental mesenchymal cells.
|
| Journal |
Stem Cells Dev
|
| Abstract |
Similar to embryonic stem cells, induced pluripotent stem (iPS) cells can differentiate into various cell types upon appropriate induction, and thus, may be valuable cell sources for regenerative medicine. However, iPS cells have not been reported to differentiate into odontogenic cells for tooth regeneration. Here we demonstrated that neural crest-like cells (NCLC) derived from mouse iPS cells have the potential to differentiate into odontogenic mesenchymal cells. We developed an efficient culture protocol to induce the differentiation of mouse iPS cells into NCLC. We confirmed that the cells exhibited neural crest (NC) cell markers as evidenced by immunocytochemistry, flow cytometry, and real-time reverse transcription-polymerase chain reaction. Further, in recombination cultures of NCLC and mouse dental epithelium, NCLC exhibited a gene expression pattern involving dental mesenchymal cells. Some NCLC also expressed dentin sialoprotein. Conditioned medium of mouse dental epithelium cultures further enhanced the differentiation of NCLC into odontoblasts. These results suggest that iPS cells are useful cell sources for tooth regeneration and tooth development studies.
|
| Volume |
21(7)
|
| Pages |
1156-64
|
| Published |
2012-5-1
|
| DOI |
10.1089/scd.2011.0210
|
| PMID |
22085204
|
| MeSH |
Animals
Biomarkers / metabolism
Cell Differentiation*
Cell Transformation, Neoplastic
Cells, Cultured
Coculture Techniques
Epithelial Cells / metabolism
Gene Expression
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / physiology*
Induced Pluripotent Stem Cells / transplantation
Mice
Mice, Nude
Neural Crest / metabolism
Odontoblasts / metabolism
Odontogenesis
Teratoma / pathology
Tooth Germ / metabolism
|
| IF |
3.153
|
| Times Cited |
65
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
HEMATOLOGY
CELL & TISSUE ENGINEERING
|
| Resource |
| Human and Animal Cells |
iPS-MEF-Ng-20D-17(APS0001) |
