| RRC ID |
18584
|
| 著者 |
Tsukasaki M, Yamada A, Suzuki D, Aizawa R, Miyazono A, Miyamoto Y, Suzawa T, Takami M, Yoshimura K, Morimura N, Yamamoto M, Kamijo R.
|
| タイトル |
Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-α.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
POEM, also known as nephronectin, is an extracellular matrix protein considered to be a positive regulator of osteoblast differentiation. In the present study, we found that tumor necrosis factor-α (TNF-α), a key regulator of bone matrix properties and composition that also inhibits terminal osteoblast differentiation, strongly inhibited POEM expression in the mouse osteoblastic cell line MC3T3-E1. TNF-α-induced down-regulation of POEM gene expression occurred in both time- and dose-dependent manners through the nuclear factor kappa B (NF-κB) pathway. In addition, expressions of marker genes in differentiated osteoblasts were down-regulated by TNF-α in a manner consistent with our findings for POEM, while over-expression of POEM recovered TNF-α-induced inhibition of osteoblast differentiation. These results suggest that TNF-α inhibits POEM expression through the NF-κB signaling pathway and down-regulation of POEM influences the inhibition of osteoblast differentiation by TNF-α.
|
| 巻・号 |
410(4)
|
| ページ |
766-70
|
| 公開日 |
2011-7-15
|
| DOI |
10.1016/j.bbrc.2011.06.048
|
| PII |
S0006-291X(11)01006-0
|
| PMID |
21689636
|
| MeSH |
Animals
Cell Differentiation / drug effects
Cell Differentiation / genetics*
Cell Line
Extracellular Matrix Proteins / antagonists & inhibitors*
Extracellular Matrix Proteins / genetics*
Gene Expression Regulation*
Humans
Mice
NF-kappa B / antagonists & inhibitors
NF-kappa B / metabolism
Osteoblasts / cytology*
Tumor Necrosis Factor-alpha / metabolism*
Tumor Necrosis Factor-alpha / pharmacology
|
| IF |
2.985
|
| 引用数 |
17
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
