論文 - 詳細
| RRC ID | 18595 |
|---|---|
| 著者 | Ogawa S, Tokumoto Y, Miyake J, Nagamune T. |
| タイトル | Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells. |
| ジャーナル | In Vitro Cell Dev Biol Anim |
| Abstract |
Induced pluripotent stem cells (iPSCs) prepared from somatic cells might become a novel therapeutic tool in regenerative medicine, especially for the central nervous system (CNS). In this study, we attempted to induce O4-positive (O4(+)) oligodendrocytes from adult human fibroblast-derived iPSCs in vitro. We used two adult human iPSC cell lines, 201B7 and 253G1. 201B7 was induced by four-gene transduction (oct4, sox2, klf4, c-myc), and 253G1 was induced by three-gene transduction (oct4, sox2, klf4). We treated these cells with two in vitro oligodendrocyte-directed differentiation protocols that were optimized for human embryonic stem cells. One protocol used platelet-derived growth factor as the major mitogen for oligodendrocyte lineage cells, and the other protocol used epidermal growth factor (EGF) as the mitogen. Although the differentiation efficiency was low (less than 0.01%), we could induce O4(+) oligodendrocytes from 253G1 cells using the EGF-dependent differentiation protocol. This is the first report of the in vitro induction of oligodendrocytes differentiation from human iPSCs. |
| 巻・号 | 47(7) |
| ページ | 464-9 |
| 公開日 | 2011-8-1 |
| DOI | 10.1007/s11626-011-9435-2 |
| PMID | 21695581 |
| MeSH | Adult Animals Cell Culture Techniques* Cell Differentiation / physiology* Cell Line Female Fibroblasts / cytology Fibroblasts / physiology* Humans Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / physiology* Kruppel-Like Factor 4 Mice Oligodendroglia / cytology Oligodendroglia / physiology* |
| IF | 1.665 |
| 引用数 | 39 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) 253G1(HPS0002) |