RRC ID 18638
Author El-Shazly AE, Moonen V, Mawet M, Begon D, Henket M, Arafa M, Louis R, Delvenne P, Lefebvre PP.
Title IFN-γ and TNF-α potentiate prostaglandin D2-induced human eosinophil chemotaxis through up-regulation of CRTH2 surface receptor.
Journal Int. Immunopharmacol.
Abstract Prostaglandin D2 (PGD2) receptor CRTH2, is a pro-inflammatory molecule involved in eosinophil recruitment to the allergic airway. We investigated the expression of CRTH2 in eosinophil from allergic rhinitis patients (AR) and tested the modulatory role of several TH1 and TH2 cytokines closely related to the allergic immunological response, on the expression of CRTH2 receptor, utilizing human eosinophil cell line (Eol-1).The expression of CRTH2 was tested by immunohistochemistry and flow cytometry (FACS). Chemotaxis was performed in micro-chemotaxis chambers. It is shown that the expression of CRTH2 by eosinophils was significantly higher in the nasal tissue and peripheral blood of AR patients, when compared to control subjects. PGD2 exhibited a typical bell shape dose response in attracting eosinophil from AR patients with optimal activity at 10(-7) M. Eol-1 cell surface expression of CRTH2 was significantly up-regulated by 10 ng/ml IFN-γ and TNF-α. The percentage of Eol-1 cells expressing the receptor increased by IFN-γ and TNF-α from 12.74%±2.66 to 55%±8 and 33.8%±9.4, respectively. PGD2-induced Eol-1 chemotaxis was not blocked by SB203580, H-89 Dihydrochloride, Bisindo-lylmaleimide, or Genistein. PGD2-induced Eol-1 chemotaxis was potentiated by IFN-γ and TNF-α without changing the signal transduction pathway. Correlation of our results to peripheral blood eosinophils from allergic rhinitis patients confirmed that 3 hour pretreatment of eosinophils by 10 ng/ml IFN-γ and TNF-α, increased the mean fluorescence intensity (MFI) of CRTH2 from 8.23 to 9.68 and 9.38, respectively, and potentiated PGD2-induced eosinophil chemotaxis. Our results demonstrate a novel synergism between PGD2, IFN-γ and TNF-α, in eosinophil chemotaxis.
Volume 11(11)
Pages 1864-70
Published 2011-11
DOI 10.1016/j.intimp.2011.07.017
PII S1567-5769(11)00294-3
PMID 21835268
MeSH Blotting, Western Case-Control Studies Cell Culture Techniques Cell Line, Tumor Chemotaxis, Leukocyte / drug effects* Dose-Response Relationship, Immunologic Eosinophils / drug effects* Eosinophils / immunology Eosinophils / metabolism Flow Cytometry Humans Immunohistochemistry Interferon-gamma / immunology Interferon-gamma / pharmacology* Nasal Mucosa / drug effects Nasal Mucosa / immunology Nasal Mucosa / pathology Prostaglandin D2 / pharmacology* Receptors, Immunologic / biosynthesis* Receptors, Immunologic / immunology Receptors, Prostaglandin / biosynthesis* Receptors, Prostaglandin / immunology Rhinitis, Allergic, Perennial / immunology Rhinitis, Allergic, Perennial / metabolism Rhinitis, Allergic, Perennial / pathology Tumor Necrosis Factor-alpha / immunology Tumor Necrosis Factor-alpha / pharmacology* Up-Regulation
IF 3.361
Times Cited 10
Human and Animal Cells EoL-1 cell (RCB0641)