RRC ID 18644
Author Endo T, Kobayashi T.
Title Thyroid-specific gene expression in chondrocytes.
Journal Biochem Biophys Res Commun
Abstract Previously, we demonstrated that Runx2 (Cbfa1/AML3), a chondrocyte-specific transcription factor, is expressed in thyroid glands of mice, where it stimulates expression of the thyroglobulin (Tg) gene. Here, we reverse transcribed thyroid transcription factor-1 (TTF-1), Pax-8, Tg, thyroid peroxidase (TPO) and Na(+)/I(-) symporter (NIS) cDNAs from mouse trachea and bronchus RNA samples, but were unable to recover these cDNAs from mouse liver RNA samples. Tg mRNA levels in trachea and bronchus were about 5.1% and 2.1% of those in thyroid glands. ATDC-5 cells, cultured chondrocytes, expressed about 30-fold more Tg mRNA than undifferentiated cells. Gel shift and Tg gene reporter assay revealed that TTF-1 stimulated Tg gene expression in these cells. These results indicate that chondrocytes turn on some aspects of the thyroid gene expression program and that TTF-1 plays important roles in Tg gene expression in chondrocyte.
Volume 416(3-4)
Pages 227-31
Published 2011-12-16
DOI 10.1016/j.bbrc.2011.08.122
PII S0006-291X(11)01541-5
PMID 21945616
MeSH Animals Cartilage / metabolism* Cells, Cultured Chondrocytes / metabolism* DNA, Complementary / genetics Gene Expression* Iodide Peroxidase / genetics Mice Nuclear Proteins / genetics Paired Box Transcription Factors / genetics Symporters / genetics Thyroglobulin / genetics Thyroid Gland / metabolism* Thyroid Nuclear Factor 1 Transcription Factors / genetics
IF 2.985
Times Cited 2
Human and Animal Cells ATDC5(RCB0565)