Reference - Detail
| RRC ID | 18649 |
|---|---|
| Author | Takegawa Y, Araki K, Fujitani N, Furukawa J, Sugiyama H, Sakai H, Shinohara Y. |
| Title | Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography. |
| Journal | Anal Chem |
| Abstract |
Glycosaminoglycans (GAGs) play important roles in cell adhesion and growth, maintenance of extracellular matrix (ECM) integrity, and signal transduction. To fully understand the biological functions of GAGs, there is a growing need for sensitive, rapid, and quantitative analysis of GAGs. The present work describes a novel analytical technique that enables high throughput cellular/tissue glycosaminoglycomics for all three families of uronic acid-containing GAGs, hyaluronan (HA), chondroitin sulfate (CS)/dermatan sulfate (DS), and heparan sulfate (HS). A one-pot purification and labeling procedure for GAG Δ-disaccharides was established by chemo-selective ligation of disaccharides onto high density hydrazide beads (glycoblotting) and subsequent labeling by fluorescence. The 17 most common disaccharides (eight comprising HS, eight CS/DS, and one comprising HA) could be separated with a single chromatography for the first time by employing a zwitter-ionic type of hydrophilic-interaction chromatography column. These novel analytical techniques were able to precisely characterize the glycosaminoglycome in various cell types including embryonal carcinoma cells and ocular epithelial tissues (cornea, conjunctiva, and limbus). |
| Volume | 83(24) |
| Pages | 9443-9 |
| Published | 2011-12-15 |
| DOI | 10.1021/ac2021079 |
| PMID | 22044073 |
| MeSH | Animals Cell Line Chondroitin Sulfates / analysis* Chromatography, High Pressure Liquid* Conjunctiva / metabolism Cricetinae Dermatan Sulfate / analysis* Disaccharides / analysis Heparitin Sulfate / analysis* Humans Hyaluronic Acid / analysis* Hydrophobic and Hydrophilic Interactions Ions / chemistry Mice Rabbits |
| IF | 6.785 |
| Times Cited | 33 |
| WOS Category | CHEMISTRY, ANALYTICAL |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CHO-K1(RCB0285) |