論文 - 詳細
| RRC ID | 18669 |
|---|---|
| 著者 | Hu R, Liu W, Li H, Yang L, Chen C, Xia ZY, Guo LJ, Xie H, Zhou HD, Wu XP, Luo XH. |
| タイトル | A Runx2/miR-3960/miR-2861 regulatory feedback loop during mouse osteoblast differentiation. |
| ジャーナル | J Biol Chem |
| Abstract |
Our recent study showed that miR-2861 promotes osteoblast differentiation by targeting histone deacetylase 5, resulting in increased runt-related transcription factor 2 (Runx2) protein production. Here we identified another new microRNA (miRNA) (miR-3960) that played a regulatory role in osteoblast differentiation through a regulatory feedback loop with miR-2861. miR-3960 and miR-2861 were found clustered at the same loci. miR-3960 was transcribed during bone morphogenic protein 2 (BMP2)-induced osteogenesis of ST2 stromal cells. Overexpression of miR-3960 promoted BMP2-induced osteoblastogenesis. However, the inhibition of miR-3960 expression attenuated the osteoblastogenesis. Homeobox A2 (Hoxa2), a repressor of Runx2 expression, was confirmed to be a target of miR-3960. Electrophoretic mobility shift assay and chromatin immunoprecipitation experiments confirmed that Runx2 bound to the promoter of the miR-3960/miR-2861 cluster. Furthermore, overexpression of Runx2 induced miR-3960/miR-2861 transcription, and block of Runx2 expression attenuated BMP2-induced miR-3960/miR-2861 transcription. Here we report that miR-3960 and miR-2861, transcribed together from the same miRNA polycistron, both function in osteoblast differentiation through a novel Runx2/miR-3960/miR-2861 regulatory feedback loop. Our findings provide new insights into the roles of miRNAs in osteoblast differentiation. |
| 巻・号 | 286(14) |
| ページ | 12328-39 |
| 公開日 | 2011-4-8 |
| DOI | 10.1074/jbc.M110.176099 |
| PII | S0021-9258(20)51612-8 |
| PMID | 21324897 |
| PMC | PMC3069436 |
| MeSH | Animals Animals, Newborn Blotting, Northern Blotting, Western Cell Differentiation / genetics Cell Differentiation / physiology Cells, Cultured Chromatin Immunoprecipitation Computational Biology Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism* Electrophoretic Mobility Shift Assay Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Mice Mice, Inbred C57BL MicroRNAs / genetics MicroRNAs / physiology* Osteoblasts / cytology* Osteoblasts / metabolism RNA, Small Interfering / genetics Reverse Transcriptase Polymerase Chain Reaction |
| IF | 4.238 |
| 引用数 | 166 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) |