| RRC ID |
18679
|
| 著者 |
Chong PK, Lee H, Zhou J, Liu SC, Loh MC, So JB, Lim KH, Yeoh KG, Lim YP.
|
| タイトル |
Reduced plasma APOA1 level is associated with gastric tumor growth in MKN45 mouse xenograft model.
|
| ジャーナル |
J Proteomics
|
| Abstract |
There is no suitable diagnostic and prognostic biomarker for gastric cancer. The biggest hurdles in biomarker discovery are (i) the low abundance of cancer cell-specific proteins that limits their detection and (ii) complex inter-patient variations that complicate the discovery process. To circumvent these issues, we conducted proteomics on the plasma of gastric cancer mouse xenograft and attempted to identify proteins released by cancer cells. MKN45 gastric cancer cells were subcutaneously implanted into immune-incompetent nude mice. Plasma samples collected from mice with different tumor sizes (low, mid and high tumor loads) were subjected to iTRAQ and mass spectrometric analyses. Detection of human APOA1 in mouse plasma was verified and its expression level was shown to be lower in mice with large tumors compared to those with small tumors. Studies on a panel of about 14 gastric cancer cell lines supported the notion that APOA1 in mouse plasma was of human gastric cancer cell origin. While the clinical utility of APOA1 remains to be ascertained with a larger scale study, the current work supported the feasibility of using mouse xenograft model for gastric cancer biomarker discovery.
|
| 巻・号 |
73(8)
|
| ページ |
1632-40
|
| 公開日 |
2010-6-16
|
| DOI |
10.1016/j.jprot.2010.04.005
|
| PII |
S1874-3919(10)00131-4
|
| PMID |
20399916
|
| MeSH |
Amino Acid Sequence
Animals
Apolipoprotein A-I / blood*
Biomarkers, Tumor / blood*
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Molecular Sequence Data
Neoplasm Transplantation
Proteomics / methods
Sequence Alignment
Stomach Neoplasms / genetics
Stomach Neoplasms / metabolism*
Xenograft Model Antitumor Assays*
|
| IF |
3.509
|
| 引用数 |
41
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001) |
