RRC ID 18680
著者 Chong PK, Lee H, Zhou J, Liu SC, Loh MC, Wang TT, Chan SP, Smoot DT, Ashktorab H, So JB, Lim KH, Yeoh KG, Lim YP.
タイトル ITIH3 is a potential biomarker for early detection of gastric cancer.
ジャーナル J Proteome Res
Abstract Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.
巻・号 9(7)
ページ 3671-9
公開日 2010-7-2
DOI 10.1021/pr100192h
PMID 20515073
PMC PMC3760204
MeSH Alpha-Globulins / analysis Alpha-Globulins / metabolism* Animals Biomarkers, Tumor / analysis Biomarkers, Tumor / metabolism* Blood Proteins / analysis Blotting, Western Cell Line, Tumor Densitometry Histocytochemistry Humans Isotope Labeling Mass Spectrometry Mice Mice, Inbred BALB C Proteome / analysis Proteome / metabolism* ROC Curve Reproducibility of Results Stomach Neoplasms / diagnosis Stomach Neoplasms / metabolism* Transplantation, Heterologous
IF 4.074
引用数 36
WOS 分野 BIOCHEMICAL RESEARCH METHODS
リソース情報
ヒト・動物細胞 MKN45(RCB1001)