| RRC ID |
18690
|
| Author |
Itoigawa Y, Kishimoto KN, Okuno H, Sano H, Kaneko K, Itoi E.
|
| Title |
Hypoxia induces adipogenic differentitation of myoblastic cell lines.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Muscle atrophy usually accompanies fat accumulation in the muscle. In such atrophic conditions as back muscles of kyphotic spine and the rotator cuff muscles with torn tendons, blood flow might be diminished. It is known that hypoxia causes trans-differentiation of mesenchymal stem cells derived from bone marrow into adipocytes. However, it has not been elucidated yet if hypoxia turned myoblasts into adipocytes. We investigated adipogenesis in C2C12 and G8 murine myogenic cell line treated by hypoxia. Cells were also treated with the cocktail of insulin, dexamethasone and IBMX (MDI), which has been known to inhibit Wnt signaling and promote adipogenesis. Adipogenic differentiation was seen in both hypoxia and MDI. Adipogenic marker gene expression was assessed in C2C12. CCAAT/enhancer-binding protein (C/EBP) beta, alpha and peroxisome proliferator activating receptor (PPAR) gamma were increased by both hypoxia and MDI. The expression profile of Wnt10b was different between hypoxia and MDI. The mechanism for adipogenesis of myoblasts in hypoxia might be regulated by different mechanism than the modification of Wnt signaling.
|
| Volume |
399(4)
|
| Pages |
721-6
|
| Published |
2010-9-3
|
| DOI |
10.1016/j.bbrc.2010.08.007
|
| PII |
S0006-291X(10)01484-1
|
| PMID |
20692234
|
| MeSH |
1-Methyl-3-isobutylxanthine / pharmacology
Adipocytes / metabolism
Adipocytes / pathology*
Adipogenesis*
Animals
CCAAT-Enhancer-Binding Protein-alpha / metabolism
CCAAT-Enhancer-Binding Protein-beta / metabolism
Cell Line
Dexamethasone / pharmacology
Gene Expression Regulation
Hypoxia / metabolism
Hypoxia / pathology*
Insulin / pharmacology
Mice
Muscular Atrophy / metabolism
Muscular Atrophy / pathology*
Myoblasts / drug effects
Myoblasts / metabolism
Myoblasts / pathology*
Nerve Tissue Proteins / genetics
PPAR gamma / metabolism
Wnt Proteins / genetics
|
| IF |
2.985
|
| Times Cited |
13
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
