RRC ID 18691
著者 Shirakura M, Tanimoto K, Eguchi H, Miyauchi M, Nakamura H, Hiyama K, Tanimoto K, Tanaka E, Takata T, Tanne K.
タイトル Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis.
ジャーナル Biochem Biophys Res Commun
Abstract Vascular endothelial growth factor (Vegf) was previously shown to be expressed specifically in the condylar cartilage of temporomandibular joint-osteoarthritis (TMJ-OA) model rats. Here we demonstrate for the first time that hypoxia-inducible factor-1alpha (Hif-1alpha) is activated in mature chondrocytes of temporomandibular joint-osteoarthritis (TMJ-OA) model rat by mechanical overload, and that activated Hif-1 in chondrocytes can induce osteoclastogenesis via repression of osteoprotegerin (Opg) expression. In rat TMJs, degeneration of the condylar cartilage became prominent in proportion to the duration of overloading. Hif-1alpha expression was observed specifically in mature and hypertrophic chondrocytes, and Hif-1alpha-positivity, level of Vegf expression, and tartrate-resistant acid phosphatase (TRAP)-positive cell numbers all increased in the same manner. When ATDC5 cells induced differentiation by insulin were cultured under hypoxia, Hif-1alpha induction was observed in mature stage, but not in immature stage. Inductions of Hif-1-target genes showed a similar expression pattern. In addition, expression of Opg decreased in hypoxia, and Hif-1alpha played a role, in part, in its regulation.
巻・号 393(4)
ページ 800-5
公開日 2010-3-19
DOI 10.1016/j.bbrc.2010.02.086
PII S0006-291X(10)00306-2
PMID 20171183
MeSH Animals Bone Resorption / metabolism Bone Resorption / pathology* Cartilage, Articular / metabolism Cartilage, Articular / pathology* Chondrocytes / metabolism Chondrocytes / pathology Disease Models, Animal Hypoxia-Inducible Factor 1, alpha Subunit / agonists* Hypoxia-Inducible Factor 1, alpha Subunit / biosynthesis Male Osteoarthritis / metabolism Osteoarthritis / pathology* Osteoclasts / metabolism Osteoclasts / pathology* Rats Rats, Wistar Temporomandibular Joint Disorders / metabolism Temporomandibular Joint Disorders / pathology*
IF 2.985
引用数 30
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)