RRC ID |
18691
|
著者 |
Shirakura M, Tanimoto K, Eguchi H, Miyauchi M, Nakamura H, Hiyama K, Tanimoto K, Tanaka E, Takata T, Tanne K.
|
タイトル |
Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Vascular endothelial growth factor (Vegf) was previously shown to be expressed specifically in the condylar cartilage of temporomandibular joint-osteoarthritis (TMJ-OA) model rats. Here we demonstrate for the first time that hypoxia-inducible factor-1alpha (Hif-1alpha) is activated in mature chondrocytes of temporomandibular joint-osteoarthritis (TMJ-OA) model rat by mechanical overload, and that activated Hif-1 in chondrocytes can induce osteoclastogenesis via repression of osteoprotegerin (Opg) expression. In rat TMJs, degeneration of the condylar cartilage became prominent in proportion to the duration of overloading. Hif-1alpha expression was observed specifically in mature and hypertrophic chondrocytes, and Hif-1alpha-positivity, level of Vegf expression, and tartrate-resistant acid phosphatase (TRAP)-positive cell numbers all increased in the same manner. When ATDC5 cells induced differentiation by insulin were cultured under hypoxia, Hif-1alpha induction was observed in mature stage, but not in immature stage. Inductions of Hif-1-target genes showed a similar expression pattern. In addition, expression of Opg decreased in hypoxia, and Hif-1alpha played a role, in part, in its regulation.
|
巻・号 |
393(4)
|
ページ |
800-5
|
公開日 |
2010-3-19
|
DOI |
10.1016/j.bbrc.2010.02.086
|
PII |
S0006-291X(10)00306-2
|
PMID |
20171183
|
MeSH |
Animals
Bone Resorption / metabolism
Bone Resorption / pathology*
Cartilage, Articular / metabolism
Cartilage, Articular / pathology*
Chondrocytes / metabolism
Chondrocytes / pathology
Disease Models, Animal
Hypoxia-Inducible Factor 1, alpha Subunit / agonists*
Hypoxia-Inducible Factor 1, alpha Subunit / biosynthesis
Male
Osteoarthritis / metabolism
Osteoarthritis / pathology*
Osteoclasts / metabolism
Osteoclasts / pathology*
Rats
Rats, Wistar
Temporomandibular Joint Disorders / metabolism
Temporomandibular Joint Disorders / pathology*
|
IF |
2.985
|
引用数 |
30
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
ATDC5(RCB0565) |