RRC ID 18693
著者 Saito Y, Sekine W, Sano R, Komatsu S, Mizuno H, Katabami K, Shimada K, Oku T, Tsuji T.
タイトル Potentiation of cell invasion and matrix metalloproteinase production by alpha3beta1 integrin-mediated adhesion of gastric carcinoma cells to laminin-5.
ジャーナル Clin Exp Metastasis
Abstract We previously reported that the adhesion of gastric carcinoma cells to the peritoneum mediated by the alpha3beta1 integrin-laminin interaction is a key step in the initial process of peritoneal metastatic dissemination. Carcinoma cells subsequently invade through the intercellular gaps of mesothelial linings. In this study, we examined the role of the interaction of carcinoma cells with laminin-5, which is a major component of submesothelial basement membranes and serves as a high-affinity ligand for alpha3beta1 integrin, in carcinoma cell invasion. Human gastric carcinoma cell lines (MKN1, GT3TKB, and NUGC-4) adhered in an alpha3beta1 integrin-dependent manner to the extracellular matrix deposited by peritoneal mesothelial cells. An in vitro invasion assay using the Boyden chamber system revealed that MKN1 cell migration through the membranes increased when the membranes were coated with matrices produced by mesothelial cells or with laminin-5-containing Matrigel as compared to Matrigel alone. The cell migration promoted by laminin-5-containing Matrigel was inhibited by the presence of anti-alpha3 integrin antibody. When MKN1 cells were cultured in a laminin-5-coated plate, these cells were promoted to produce matrix metalloproteinase (MMP)-9, as assessed by gelatin zymography, enzyme-linked immunosorbent assay, and reverse transcription-polymerase chain reaction. These results suggest that the production of MMP-9 by MKN1 cells was potentiated by the alpha3beta1 integrin-laminin-5 interaction, which facilitated their invasion via degradation of the matrix.
巻・号 27(4)
ページ 197-205
公開日 2010-4-1
DOI 10.1007/s10585-010-9314-3
PMID 20352300
MeSH Animals Cell Adhesion Cell Adhesion Molecules / metabolism* Cell Line, Tumor Cell Movement Humans Integrin alpha3beta1 / metabolism* Matrix Metalloproteinase 9 / biosynthesis* Matrix Metalloproteinase 9 / genetics Matrix Metalloproteinase 9 / metabolism Mice Mice, Inbred ICR Peritoneum / cytology Peritoneum / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Stomach Neoplasms / enzymology Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology*
IF 3.027
引用数 28
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MKN1(RCB1003) MKN45(RCB1001) GT3TKB(RCB0885)