論文 - 詳細
| RRC ID | 18710 |
|---|---|
| 著者 | Usui Y, Uematsu T, Uchihashi T, Takahashi M, Takahashi M, Ishizuka M, Doto R, Tanaka H, Komazaki Y, Osawa M, Yamada K, Yamaoka M, Furusawa K. |
| タイトル | Inorganic polyphosphate induces osteoblastic differentiation. |
| ジャーナル | J Dent Res |
| Abstract |
Inorganic polyphosphate [Poly(P)] is especially prevalent in osteoblasts. We tested the hypothesis that Poly(P) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. The osteoblast-like cell line, MC 3T3-E1, was cultured with Poly(P), and gene expression was evaluated by real-time reverse-transcription polymerase chain-reaction. Phosphatase activity and extracellular matrix mineralization were also determined. The role of Poly(P) was assessed in a beagle dog alveolar bone regeneration model. Poly(P) increased osteocalcin, osterix, bone sialoprotein, and tissue non-specific alkaline phosphatase gene expression, with a high level of end-polyphosphatase activity, resulting in low-chain-length Poly(P), inorganic pyrophosphate, and inorganic phosphate production. MC3T3-E1 cells differentiated into mature osteoblasts and showed expression of ectonucleotide pyrophosphatase phosphodiesterase 1, while mouse progressive ankylosis gene expression remained unchanged. Promotion of alveolar bone regeneration was observed in Poly(P)-treated beagle dogs. These findings suggest that Poly(P) induces osteoblastic differentiation and bone mineralization, and acts as a resource for mineralization. |
| 巻・号 | 89(5) |
| ページ | 504-9 |
| 公開日 | 2010-5-1 |
| DOI | 10.1177/0022034510363096 |
| PII | 0022034510363096 |
| PMID | 20332330 |
| MeSH | 3T3 Cells Acid Anhydride Hydrolases / analysis Alkaline Phosphatase / analysis Alveolar Bone Loss / surgery Alveolar Process / drug effects Animals Bone Regeneration / drug effects Calcification, Physiologic / drug effects Cell Differentiation / drug effects Dental Enamel Proteins / therapeutic use Diphosphates / analysis Dogs Extracellular Matrix / drug effects Furcation Defects / surgery Integrin-Binding Sialoprotein Male Mice Osteoblasts / drug effects* Osteocalcin / analysis Phenotype Phosphates / analysis Phosphoric Diester Hydrolases / analysis Polyphosphates / pharmacology* Polyphosphates / therapeutic use Pyrophosphatases / analysis Sialoglycoproteins / analysis Sp7 Transcription Factor Transcription Factors / analysis Zinc Fingers / drug effects |
| IF | 4.914 |
| 引用数 | 40 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |