RRC ID 18710
Author Usui Y, Uematsu T, Uchihashi T, Takahashi M, Takahashi M, Ishizuka M, Doto R, Tanaka H, Komazaki Y, Osawa M, Yamada K, Yamaoka M, Furusawa K.
Title Inorganic polyphosphate induces osteoblastic differentiation.
Journal J Dent Res
Abstract Inorganic polyphosphate [Poly(P)] is especially prevalent in osteoblasts. We tested the hypothesis that Poly(P) stimulates osteoblastic differentiation and polyphosphate metabolism for bone formation. The osteoblast-like cell line, MC 3T3-E1, was cultured with Poly(P), and gene expression was evaluated by real-time reverse-transcription polymerase chain-reaction. Phosphatase activity and extracellular matrix mineralization were also determined. The role of Poly(P) was assessed in a beagle dog alveolar bone regeneration model. Poly(P) increased osteocalcin, osterix, bone sialoprotein, and tissue non-specific alkaline phosphatase gene expression, with a high level of end-polyphosphatase activity, resulting in low-chain-length Poly(P), inorganic pyrophosphate, and inorganic phosphate production. MC3T3-E1 cells differentiated into mature osteoblasts and showed expression of ectonucleotide pyrophosphatase phosphodiesterase 1, while mouse progressive ankylosis gene expression remained unchanged. Promotion of alveolar bone regeneration was observed in Poly(P)-treated beagle dogs. These findings suggest that Poly(P) induces osteoblastic differentiation and bone mineralization, and acts as a resource for mineralization.
Volume 89(5)
Pages 504-9
Published 2010-5-1
DOI 10.1177/0022034510363096
PII 0022034510363096
PMID 20332330
MeSH 3T3 Cells Acid Anhydride Hydrolases / analysis Alkaline Phosphatase / analysis Alveolar Bone Loss / surgery Alveolar Process / drug effects Animals Bone Regeneration / drug effects Calcification, Physiologic / drug effects Cell Differentiation / drug effects Dental Enamel Proteins / therapeutic use Diphosphates / analysis Dogs Extracellular Matrix / drug effects Furcation Defects / surgery Integrin-Binding Sialoprotein Male Mice Osteoblasts / drug effects* Osteocalcin / analysis Phenotype Phosphates / analysis Phosphoric Diester Hydrolases / analysis Polyphosphates / pharmacology* Polyphosphates / therapeutic use Pyrophosphatases / analysis Sialoglycoproteins / analysis Sp7 Transcription Factor Transcription Factors / analysis Zinc Fingers / drug effects
IF 4.914
Times Cited 40
Human and Animal Cells MC3T3-E1(RCB1126)