RRC ID 18718
著者 Yasuzaki Y, Yamada Y, Harashima H.
タイトル Mitochondrial matrix delivery using MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes.
ジャーナル Biochem Biophys Res Commun
Abstract Mitochondria are the principal producers of energy in cells of higher organisms. It was recently reported that mutations and defects in mitochondrial DNA (mtDNA) are associated with various mitochondrial diseases including a variety of neurodegenerative and neuromuscular diseases. Therefore, an effective mitochondrial gene therapy and diagnosis would be expected to have great medical benefits. To achieve this, therapeutic agents need to be delivered into the innermost mitochondrial space (mitochondrial matrix), which contains the mtDNA pool. We previously reported on the development of MITO-Porter, a liposome-based carrier that introduces macromolecular cargos into mitochondria via membrane fusion. In this study, we provide a demonstration of mitochondrial matrix delivery and the visualization of mitochondrial genes (mtDNA) in living cells using the MITO-Porter. We first prepared MITO-Porter containing encapsulated propidium iodide (PI), a fluorescent dye used to stain nucleic acids to detect mtDNA. We then confirmed the emission of red-fluorescence from PI by conjugation with mtDNA, when the carriers were incubated in the presence of isolated rat liver mitochondria. Finally, intracellular observation by confocal laser scanning microscopy clearly verified that the MITO-Porter delivered PI to the mitochondrial matrix.
巻・号 397(2)
ページ 181-6
公開日 2010-6-25
DOI 10.1016/j.bbrc.2010.05.070
PII S0006-291X(10)00961-7
PMID 20580633
MeSH Animals DNA, Mitochondrial / analysis DNA, Mitochondrial / metabolism* Fluorescent Dyes / analysis Fluorescent Dyes / metabolism* Genetic Therapy* HeLa Cells Humans Liposomes / analysis Liposomes / metabolism* Membrane Fusion Microscopy, Confocal Mitochondria, Liver / chemistry Mitochondria, Liver / metabolism* Mitochondrial Diseases / therapy Mitochondrial Membranes / chemistry Mitochondrial Membranes / metabolism* Propidium / analysis Propidium / metabolism* Rats Rats, Wistar Transduction, Genetic*
IF 2.985
引用数 40
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HeLa(RCB0007)