RRC ID 1872
Author Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL.
Title Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis.
Journal Plant Physiol
Abstract Here, we describe how the semidominant, gain-of-function icu4-1 and icu4-2 alleles of the INCURVATA4 (ICU4) gene alter leaf phyllotaxis and cell organization in the root apical meristem, reduce root length, and cause xylem overgrowth in the stem. The ICU4 gene was positionally cloned and found to encode the ATHB15 transcription factor, a class III homeodomain/leucine zipper family member, recently named CORONA. The icu4-1 and icu4-2 alleles bear the same point mutation that affects the microRNA complementarity site of ICU4 and is identical to those of several semidominant alleles of the class III homeodomain/leucine zipper family members PHABULOSA and PHAVOLUTA. The icu4-1 and icu4-2 mutations significantly increase leaf transcript levels of the ICU4 gene. The null hst-1 allele of the HASTY gene, which encodes a nucleocytoplasmic transporter, synergistically interacts with icu4-1, the double mutant displaying partial adaxialization of rosette leaves and carpels. Our results suggest that the ICU4 gene has an adaxializing function and that it is down-regulated by microRNAs that require the HASTY protein for their biogenesis.
Volume 141(2)
Pages 607-19
Published 2006-6-1
DOI 10.1104/pp.106.077149
PII pp.106.077149
PMID 16617092
PMC PMC1475466
MeSH Alleles Amino Acid Sequence Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Base Sequence Cloning, Molecular Genes, Plant Meristem / physiology* MicroRNAs / genetics* Molecular Sequence Data Mutation* Plants, Genetically Modified Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
IF 6.305
Times Cited 68
Arabidopsis / Cultured plant cells, genes pda06113