| RRC ID |
18723
|
| 著者 |
Koshimizu TA, Tsuchiya H, Tsuda H, Fujiwara Y, Shibata K, Hirasawa A, Tsujimoto G, Fujimura A.
|
| タイトル |
Inhibition of heat shock protein 90 attenuates adenylate cyclase sensitization after chronic morphine treatment.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Cellular adaptations to chronic opioid treatment result in enhanced responsiveness of adenylate cyclase and an increase in forskolin- or agonist-stimulated cAMP production. It is, however, not known whether chaperone molecules such as heat shock proteins contribute to this adenylate cyclase sensitization. Here, we report that treatment of cells with geldanamycin, an inhibitor of heat shock protein 90 (Hsp90), led to effective attenuation of morphine-induced adenylate cyclase sensitization. In SK-N-SH human neuroblastoma cells, morphine significantly increased RNA transcript and protein levels of type I adenylate cyclase, leading to sensitization. Whole-genome tiling array analysis revealed that cAMP response element-binding protein, an important mediator for cellular adaptation to morphine, associated with the proximal promoter of Hsp90AB1 not only in SK-N-SH cells but also in rat PC12 and human embryonic kidney cells. Hsp90AB1 transcript and protein levels increased significantly during morphine treatment, and co-application of geldanamycin (0.1-10 nM) effectively suppressed the increase in forskolin-activated adenylate cyclase activation by 56%. Type I adenylate cyclase, but not Hsp90AB1, underwent significant degradation during geldanamycin treatment. These results indicate that Hsp90 is a new pharmacological target for the suppression of adenylate cyclase sensitization induced by chronic morphine treatment.
|
| 巻・号 |
392(4)
|
| ページ |
603-7
|
| 公開日 |
2010-2-19
|
| DOI |
10.1016/j.bbrc.2010.01.089
|
| PII |
S0006-291X(10)00138-5
|
| PMID |
20100459
|
| MeSH |
Adenylyl Cyclases / biosynthesis*
Adenylyl Cyclases / genetics
Animals
Benzoquinones / pharmacology
Cell Line, Tumor
Drug Tolerance*
HSP90 Heat-Shock Proteins / antagonists & inhibitors*
HSP90 Heat-Shock Proteins / metabolism
Humans
Lactams, Macrocyclic / pharmacology
Morphine / pharmacology*
Narcotics / pharmacology*
Rats
Receptors, Opioid, mu / genetics
|
| IF |
2.985
|
| 引用数 |
15
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
SK-N-SH(RCB0426) |
