Reference - Detail
| RRC ID | 18735 |
|---|---|
| Author | Sugawara Y, Matsumura T, Takegahara Y, Jin Y, Tsukasaki Y, Takeichi M, Fujinaga Y. |
| Title | Botulinum hemagglutinin disrupts the intercellular epithelial barrier by directly binding E-cadherin. |
| Journal | J Cell Biol |
| Abstract |
Botulinum neurotoxin is produced by Clostridium botulinum and forms large protein complexes through associations with nontoxic components. We recently found that hemagglutinin (HA), one of the nontoxic components, disrupts the intercellular epithelial barrier; however, the mechanism underlying this phenomenon is not known. In this study, we identified epithelial cadherin (E-cadherin) as a target molecule for HA. HA directly binds E-cadherin and disrupts E-cadherin-mediated cell to cell adhesion. Although HA binds human, bovine, and mouse E-cadherin, it does not bind rat or chicken E-cadherin homologues. HA does not interact with other members of the classical cadherin family such as neural and vascular endothelial cadherin. Expression of rat E-cadherin but not mouse rescues Madin-Darby canine kidney cells from HA-induced tight junction (TJ) disruptions. These data demonstrate that botulinum HA directly binds E-cadherin and disrupts E-cadherin-mediated cell to cell adhesion in a species-specific manner and that the HA-E-cadherin interaction is essential for the disruption of TJ function. |
| Volume | 189(4) |
| Pages | 691-700 |
| Published | 2010-5-17 |
| DOI | 10.1083/jcb.200910119 |
| PII | jcb.200910119 |
| PMID | 20457762 |
| PMC | PMC2872904 |
| MeSH | Animals Botulinum Toxins / metabolism* Botulinum Toxins / pharmacology Cadherins / chemistry Cadherins / metabolism Cattle Cell Adhesion / drug effects Cell Line Dogs Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / metabolism Hemagglutinins / metabolism* Hemagglutinins / pharmacology Humans Mice Rats |
| IF | 8.811 |
| Times Cited | 67 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 62 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MDBK(RCB0180) NBT-T1(RCB1369) |