論文 - 詳細
| RRC ID | 18739 |
|---|---|
| 著者 | Fujiwara S, Nawa A, Luo C, Kamakura M, Goshima F, Kondo C, Kiyono T, Kikkawa F, Nishiyama Y. |
| タイトル | Carrier cell-based delivery of replication-competent HSV-1 mutants enhances antitumor effect for ovarian cancer. |
| ジャーナル | Cancer Gene Ther |
| Abstract |
Oncolytic viruses capable of tumor-selective replication and cytolysis have shown early promise as cancer therapeutics. We have developed replication-competent attenuated herpes simplex virus type 1 (HSV-1) mutants, named HF10 and Hh101, which have been evaluated for their oncolytic activities. However, the host immune system remains a significant obstacle to effective intraperitoneal administration of these viruses in the clinical setting. In this study, we investigated the use of these HSV-1 mutants as oncolytic agents against ovarian cancer and the use of human peritoneal mesothelial cells (MCs) as carrier cells for intraperitoneal therapy. MCs were efficiently infected with HSV-1 mutants, and MCs loaded with HSV-1 mutants caused cell killing adequately when cocultured with cancer cells in the presence or absence of HSV antibodies. In a mouse xenograft model of ovarian cancer, the injection of infected carrier cells led to a significant reduction of tumor volume and prolonged survival in comparison with the injection of virus alone. Our results indicate that replication-competent attenuated HSV-1 exerts a potent oncolytic effect on ovarian cancer, which may be further enhanced by the utilization of a carrier cell delivery system, based on amplification of viral load and possibly on avoidance of neutralizing antibodies. |
| 巻・号 | 18(2) |
| ページ | 77-86 |
| 公開日 | 2011-2-1 |
| DOI | 10.1038/cgt.2010.53 |
| PII | cgt201053 |
| PMID | 20885447 |
| PMC | PMC3025316 |
| MeSH | Animals Cell Survival / genetics Female Herpesvirus 1, Human / genetics* Herpesvirus 1, Human / physiology Humans Mice Mice, Inbred BALB C Mice, Nude Oncolytic Virotherapy / methods* Oncolytic Viruses / genetics* Ovarian Neoplasms / genetics Ovarian Neoplasms / pathology Ovarian Neoplasms / therapy* Ovarian Neoplasms / virology Tumor Cells, Cultured Virus Replication Xenograft Model Antitumor Assays |
| IF | 4.534 |
| 引用数 | 21 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |