Reference - Detail
| RRC ID | 18745 |
|---|---|
| Author | Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J. |
| Title | Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration. |
| Journal | Methods Mol Biol |
| Abstract |
Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening. |
| Volume | 629 |
| Pages | 193-203 |
| Published | 2010-1-1 |
| DOI | 10.1007/978-1-60761-657-3_13 |
| PMID | 20387151 |
| MeSH | Biological Assay Cell Movement / drug effects Cell Movement / genetics* Collagen Type I / pharmacology Fibronectins / metabolism HeLa Cells High-Throughput Screening Assays / methods* Humans Oligonucleotide Array Sequence Analysis / methods* Phenotype Rhodamines / metabolism Statistics as Topic Transfection / methods* |
| Times Cited | 3 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | NBT-L2b(RCB1373) |