| RRC ID |
18745
|
| Author |
Onuki-Nagasaki R, Nagasaki A, Hakamada K, Uyeda TQ, Fujita S, Miyake M, Miyake J.
|
| Title |
Transfection microarrays for high-throughput phenotypic screening of genes involved in cell migration.
|
| Journal |
Methods Mol Biol
|
| Abstract |
Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
|
| Volume |
629
|
| Pages |
193-203
|
| Published |
2010-1-1
|
| DOI |
10.1007/978-1-60761-657-3_13
|
| PMID |
20387151
|
| MeSH |
Biological Assay
Cell Movement / drug effects
Cell Movement / genetics*
Collagen Type I / pharmacology
Fibronectins / metabolism
HeLa Cells
High-Throughput Screening Assays / methods*
Humans
Oligonucleotide Array Sequence Analysis / methods*
Phenotype
Rhodamines / metabolism
Statistics as Topic
Transfection / methods*
|
| Times Cited |
3
|
| Resource |
| Human and Animal Cells |
NBT-L2b(RCB1373) |
