論文 - 詳細
| RRC ID | 1878 |
|---|---|
| 著者 | Katoh A, Uenohara K, Akita M, Hashimoto T. |
| タイトル | Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid. |
| ジャーナル | Plant Physiol |
| Abstract |
NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize quinolinate from tryptophan, whereas other bacteria synthesize quinolinate from aspartate (Asp) using L-Asp oxidase and quinolinate synthase. We show here that Arabidopsis (Arabidopsis thaliana) uses the Asp-to-quinolinate pathway. The Arabidopsis L-Asp oxidase or quinolinate synthase gene complemented the Escherichia coli mutant defective in the corresponding gene, and T-DNA-based disruption of either of these genes, as well as of the gene coding for the enzyme quinolinate phosphoribosyltransferase, was embryo lethal. An analysis of functional green fluorescent protein-fused constructs and in vitro assays of uptake into isolated chloroplasts demonstrated that these three enzymes are located in the plastid. |
| 巻・号 | 141(3) |
| ページ | 851-7 |
| 公開日 | 2006-7-1 |
| DOI | 10.1104/pp.106.081091 |
| PII | pp.106.081091 |
| PMID | 16698895 |
| PMC | PMC1489895 |
| MeSH | Amino Acid Oxidoreductases / metabolism Amino Acid Oxidoreductases / physiology Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Aspartic Acid / metabolism* Escherichia coli / genetics Escherichia coli Proteins Multienzyme Complexes / metabolism Multienzyme Complexes / physiology NAD / biosynthesis* Pentosyltransferases / metabolism Pentosyltransferases / physiology Plastids / metabolism* Quinolinic Acid / metabolism |
| IF | 6.902 |
| 引用数 | 112 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda01728 pda01949 |