RRC ID 1879
Author Fukusaki E, Jumtee K, Bamba T, Yamaji T, Kobayashi A.
Title Metabolic fingerprinting and profiling of Arabidopsis thaliana leaf and its cultured cells T87 by GC/MS.
Journal Z Naturforsch C J Biosci
Abstract Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites extracted from the leaves of Arabidopsis thaliana and cultured cells from an Arabidopsis suspension culture (cell line T87) was performed. The results suggest that although cell suspension cultures and Arabidopsis leaves showed similarities in the common primary metabolite profile, nonetheless, moderate differences in quantitative profile were revealed.
Volume 61(3-4)
Pages 267-72
Published 2006-3-1
DOI 10.1515/znc-2006-3-419
PMID 16729588
MeSH Amino Acids / analysis Arabidopsis / chemistry* Arabidopsis / cytology Automation Cell Line Cells, Cultured DNA Fingerprinting / methods* Gene Expression Profiling / methods* Mass Spectrometry Monosaccharides / chemistry Plant Leaves / chemistry*
IF 1.238
Times Cited 14
Arabidopsis / Cultured plant cells, genes rpc00008