RRC ID 188
著者 Shinoda Y, Kamikubo Y, Egashira Y, Tominaga-Yoshino K, Ogura A.
タイトル Repetition of mGluR-dependent LTD causes slowly developing persistent reduction in synaptic strength accompanied by synapse elimination.
ジャーナル Brain Res
Abstract Synaptic plasticity, the cellular basis of memory, operates in a bidirectional manner. LTP (long-term potentiation) is followed by structural changes that may lead to the formation of new synapses. However, little is known whether LTD (long-term depression) is followed by morphological changes. Here we show that the repetitive induction of metabotropic glutamate receptor (mGluR)-dependent LTD in stable cultures of rat hippocampal slices led to a slowly developing persistent (ranging over weeks) reduction in synaptic strength that was accompanied by the loss of synaptic structures. LTD was induced pharmacologically 1-3 times at 24-h intervals by applying aseptically ACPD (1-aminocyclopentane-1,3-dicarboxylic acid), an agonist of group I/II mGluR, and APV (2-amino-5-phosphonovalerate), an antagonist of the NMDA (N-methyl-D-aspartate) receptor. One ACPD/APV application induced LTD that lasted less than 24 h. After three LTD inductions, however, a gradual attenuation of the fEPSP (field excitatory postsynaptic potential) amplitude and a decrease in the number of pre- and postsynaptic structures were observed. The blockade of LTD by an mGluR antagonist or a protein phosphatase 2B inhibitor abolished the development of the synaptic attenuation. In contrast to our previous finding that the repetitive LTP induction triggered a slowly developing persistent synaptic enhancement, the incremental and decremental forms of synaptic plasticity appeared to occur symmetrically not only on the minutes-hours time order but also on the days-weeks time order.
巻・号 1042(1)
ページ 99-107
公開日 2005-4-25
DOI 10.1016/j.brainres.2005.02.028
PII S0006-8993(05)00296-9
PMID 15823258
MeSH Animals Excitatory Postsynaptic Potentials / physiology* Hippocampus / metabolism* Hippocampus / ultrastructure Long-Term Synaptic Depression / physiology* Neurons / metabolism* Neurons / ultrastructure Organ Culture Techniques Rats Receptors, Metabotropic Glutamate / metabolism* Synapses / metabolism* Synapses / ultrastructure Time Factors
IF 2.733
引用数 38
WOS 分野 NEUROSCIENCES
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