RRC ID 1882
著者 Tavladoraki P, Rossi MN, Saccuti G, Perez-Amador MA, Polticelli F, Angelini R, Federico R.
タイトル Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversion.
ジャーナル Plant Physiol
Abstract Polyamine oxidase (PAO) is a flavin adenine dinucleotide-dependent enzyme involved in polyamine catabolism. Animal PAOs oxidize spermine (Spm), spermidine (Spd), and/or their acetyl derivatives to produce H2O2, an aminoaldehyde, and Spd or putrescine, respectively, thus being involved in a polyamine back-conversion pathway. On the contrary, plant PAOs that have been characterized to date oxidize Spm and Spd to produce 1,3-diaminopropane, H2O2, and an aminoaldehyde and are therefore involved in the terminal catabolism of polyamines. A database search within the Arabidopsis (Arabidopsis thaliana) genome sequence showed the presence of a gene (AtPAO1) encoding for a putative PAO with 45% amino acid sequence identity with maize (Zea mays) PAO. The AtPAO1 cDNA was isolated and cloned in a vector for heterologous expression in Escherichia coli. The recombinant protein was purified by affinity chromatography on guazatine-Sepharose 4B and was shown to be a flavoprotein able to oxidize Spm, norspermine, and N1-acetylspermine with a pH optimum at 8.0. Analysis of the reaction products showed that AtPAO1 produces Spd from Spm and norspermidine from norspermine, demonstrating a substrate oxidation mode similar to that of animal PAOs. To our knowledge, AtPAO1 is the first plant PAO reported to be involved in a polyamine back-conversion pathway.
巻・号 141(4)
ページ 1519-32
公開日 2006-8-1
DOI 10.1104/pp.106.080911
PII pp.106.080911
PMID 16778015
PMC PMC1533960
MeSH Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Cloning, Molecular DNA, Complementary / analysis Escherichia coli / genetics Hydrogen-Ion Concentration Molecular Sequence Data Oxidoreductases Acting on CH-NH Group Donors / chemistry Oxidoreductases Acting on CH-NH Group Donors / genetics Oxidoreductases Acting on CH-NH Group Donors / metabolism* Recombinant Fusion Proteins / metabolism Sequence Alignment Spermidine / analogs & derivatives Spermidine / metabolism Spermine / analogs & derivatives Spermine / metabolism
IF 6.902
引用数 107
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda11656