Reference - Detail
| RRC ID | 1882 |
|---|---|
| Author | Tavladoraki P, Rossi MN, Saccuti G, Perez-Amador MA, Polticelli F, Angelini R, Federico R. |
| Title | Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversion. |
| Journal | Plant Physiol |
| Abstract |
Polyamine oxidase (PAO) is a flavin adenine dinucleotide-dependent enzyme involved in polyamine catabolism. Animal PAOs oxidize spermine (Spm), spermidine (Spd), and/or their acetyl derivatives to produce H2O2, an aminoaldehyde, and Spd or putrescine, respectively, thus being involved in a polyamine back-conversion pathway. On the contrary, plant PAOs that have been characterized to date oxidize Spm and Spd to produce 1,3-diaminopropane, H2O2, and an aminoaldehyde and are therefore involved in the terminal catabolism of polyamines. A database search within the Arabidopsis (Arabidopsis thaliana) genome sequence showed the presence of a gene (AtPAO1) encoding for a putative PAO with 45% amino acid sequence identity with maize (Zea mays) PAO. The AtPAO1 cDNA was isolated and cloned in a vector for heterologous expression in Escherichia coli. The recombinant protein was purified by affinity chromatography on guazatine-Sepharose 4B and was shown to be a flavoprotein able to oxidize Spm, norspermine, and N1-acetylspermine with a pH optimum at 8.0. Analysis of the reaction products showed that AtPAO1 produces Spd from Spm and norspermidine from norspermine, demonstrating a substrate oxidation mode similar to that of animal PAOs. To our knowledge, AtPAO1 is the first plant PAO reported to be involved in a polyamine back-conversion pathway. |
| Volume | 141(4) |
| Pages | 1519-32 |
| Published | 2006-8-1 |
| DOI | 10.1104/pp.106.080911 |
| PII | pp.106.080911 |
| PMID | 16778015 |
| PMC | PMC1533960 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Cloning, Molecular DNA, Complementary / analysis Escherichia coli / genetics Hydrogen-Ion Concentration Molecular Sequence Data Oxidoreductases Acting on CH-NH Group Donors / chemistry Oxidoreductases Acting on CH-NH Group Donors / genetics Oxidoreductases Acting on CH-NH Group Donors / metabolism* Recombinant Fusion Proteins / metabolism Sequence Alignment Spermidine / analogs & derivatives Spermidine / metabolism Spermine / analogs & derivatives Spermine / metabolism |
| IF | 6.902 |
| Times Cited | 107 |
| WOS Category | PLANT SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda11656 |