論文 - 詳細
| RRC ID | 1882 |
|---|---|
| 著者 | Tavladoraki P, Rossi MN, Saccuti G, Perez-Amador MA, Polticelli F, Angelini R, Federico R. |
| タイトル | Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversion. |
| ジャーナル | Plant Physiol |
| Abstract |
Polyamine oxidase (PAO) is a flavin adenine dinucleotide-dependent enzyme involved in polyamine catabolism. Animal PAOs oxidize spermine (Spm), spermidine (Spd), and/or their acetyl derivatives to produce H2O2, an aminoaldehyde, and Spd or putrescine, respectively, thus being involved in a polyamine back-conversion pathway. On the contrary, plant PAOs that have been characterized to date oxidize Spm and Spd to produce 1,3-diaminopropane, H2O2, and an aminoaldehyde and are therefore involved in the terminal catabolism of polyamines. A database search within the Arabidopsis (Arabidopsis thaliana) genome sequence showed the presence of a gene (AtPAO1) encoding for a putative PAO with 45% amino acid sequence identity with maize (Zea mays) PAO. The AtPAO1 cDNA was isolated and cloned in a vector for heterologous expression in Escherichia coli. The recombinant protein was purified by affinity chromatography on guazatine-Sepharose 4B and was shown to be a flavoprotein able to oxidize Spm, norspermine, and N1-acetylspermine with a pH optimum at 8.0. Analysis of the reaction products showed that AtPAO1 produces Spd from Spm and norspermidine from norspermine, demonstrating a substrate oxidation mode similar to that of animal PAOs. To our knowledge, AtPAO1 is the first plant PAO reported to be involved in a polyamine back-conversion pathway. |
| 巻・号 | 141(4) |
| ページ | 1519-32 |
| 公開日 | 2006-8-1 |
| DOI | 10.1104/pp.106.080911 |
| PII | pp.106.080911 |
| PMID | 16778015 |
| PMC | PMC1533960 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence Cloning, Molecular DNA, Complementary / analysis Escherichia coli / genetics Hydrogen-Ion Concentration Molecular Sequence Data Oxidoreductases Acting on CH-NH Group Donors / chemistry Oxidoreductases Acting on CH-NH Group Donors / genetics Oxidoreductases Acting on CH-NH Group Donors / metabolism* Recombinant Fusion Proteins / metabolism Sequence Alignment Spermidine / analogs & derivatives Spermidine / metabolism Spermine / analogs & derivatives Spermine / metabolism |
| IF | 6.902 |
| 引用数 | 107 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda11656 |