RRC ID 1883
著者 Mo X, Zhu Q, Li X, Li J, Zeng Q, Rong H, Zhang H, Wu P.
タイトル The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance.
ジャーナル Plant Physiol
Abstract Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, we present evidence for such a role of this amino acid in root development in Arabidopsis (Arabidopsis thaliana) from the characterization of a novel Arabidopsis mutant, hpa1, which has a very short root system and carries a mutation in one of the two Arabidopsis histidinol-phosphate aminotransferase (HPA) genes, AtHPA1. We have established that AtHPA1 encodes a functional HPA and that its complete knockout is embryo lethal. Biochemical analysis shows that the mutation in hpa1 only resulted in a 30% reduction in free His content and had no significant impact on the total His content. It did not cause any known symptoms of His starvation. However, the mutant displayed a specific developmental defect in root meristem maintenance and was unable to sustain primary root growth 2 d after germination. We have demonstrated that the root meristem failure in the mutant is tightly linked to the reduction in free His content and could be rescued by either exogenous His supplementation or AtHPA1 overexpression. Our results therefore reveal an important role of His homeostasis in plant development.
巻・号 141(4)
ページ 1425-35
公開日 2006-8-1
DOI 10.1104/pp.106.084178
PII pp.106.084178
PMID 16815950
PMC PMC1533942
MeSH Amino Acid Sequence Arabidopsis / enzymology Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology* Histidine / metabolism* Histidine / physiology Homeostasis Meristem / cytology Meristem / growth & development* Meristem / metabolism Molecular Sequence Data Mutation Plant Roots / cytology Plant Roots / growth & development Plant Roots / metabolism RNA, Messenger / metabolism Seeds / anatomy & histology Seeds / growth & development Seeds / metabolism Sequence Alignment Transaminases / genetics Transaminases / metabolism Transaminases / physiology*
IF 6.902
引用数 29
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda04860