RRC ID 1884
Author Nakano T, Suzuki K, Ohtsuki N, Tsujimoto Y, Fujimura T, Shinshi H.
Title Identification of genes of the plant-specific transcription-factor families cooperatively regulated by ethylene and jasmonate in Arabidopsis thaliana.
Journal J. Plant Res.
Abstract The analysis of expression patterns of transcription-factor genes will be the basis for a better understanding of their biological functions in plants. In this study, we designed and developed an oligo-DNA macroarray consisting of gene-specific probes of 60-65 nucleotides for 288 transcription-factor genes, which cover COL, DOF, ERF, and NAC family genes. To investigate transcription-factor genes that are cooperatively regulated by jasmonate and ethylene in arabidopsis (Arabidopsis thaliana (L.) Heynh.) plants, we analyzed the expression profile of transcription-factor genes using the oligo-DNA macroarray technique in arabidopsis plants treated with methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid. Then, transcript levels of candidate genes-which were selected based on the result of macroarray analysis-were evaluated by the quantitative real-time RT-PCR method. Finally, we identified an ERF family gene that is cooperatively regulated by both hormones, and designated as cooperatively regulated by ethylene and jasmonate 1 (CEJ1).
Volume 119(4)
Pages 407-13
Published 2006-7
DOI 10.1007/s10265-006-0287-x
PMID 16820983
MeSH Arabidopsis / drug effects* Arabidopsis / genetics* Cyclopentanes / pharmacology* Ethylenes / pharmacology* Gene Expression Regulation, Plant / drug effects* Oligonucleotide Array Sequence Analysis Oxylipins Time Factors Transcription Factors / genetics* Up-Regulation / drug effects
IF 2.082
Times Cited 33
Arabidopsis / Cultured plant cells, genes pda01420 pda02979 pda06430 pda05181