論文 - 詳細
| RRC ID | 1889 |
|---|---|
| 著者 | Goepfert S, Hiltunen JK, Poirier Y. |
| タイトル | Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana. |
| ジャーナル | J Biol Chem |
| Abstract |
A gene, named AtECH2, has been identified in Arabidopsis thaliana to encode a monofunctional peroxisomal enoyl-CoA hydratase 2. Homologues of AtECH2 are present in several angiosperms belonging to the Monocotyledon and Dicotyledon classes, as well as in a gymnosperm. In vitro enzyme assays demonstrated that AtECH2 catalyzed the reversible conversion of 2E-enoyl-CoA to 3R-hydroxyacyl-CoA. AtECH2 was also demonstrated to have enoyl-CoA hydratase 2 activity in an in vivo assay relying on the synthesis of polyhydroxyalkanoate from the polymerization of 3R-hydroxyacyl-CoA in the peroxisomes of Saccharomyces cerevisiae. AtECH2 contained a peroxisome targeting signal at the C-terminal end, was addressed to the peroxisome in S. cerevisiae, and a fusion protein between AtECH2 and a fluorescent protein was targeted to peroxisomes in onion cells. AtECH2 gene expression was strongest in tissues with high beta-oxidation activity, such as germinating seedlings and senescing leaves. The contribution of AtECH2 to the degradation of unsaturated fatty acids was assessed by analyzing the carbon flux through the beta-oxidation cycle in plants that synthesize peroxisomal polyhydroxyalkanoate and that were over- or underexpressing the AtECH2 gene. These studies revealed that AtECH2 participates in vivo to the conversion of the intermediate 3R-hydroxyacyl-CoA, generated by the metabolism of fatty acids with a cis (Z)-unsaturated bond on an even-numbered carbon, to the 2E-enoyl-CoA for further degradation through the core beta-oxidation cycle. |
| 巻・号 | 281(47) |
| ページ | 35894-903 |
| 公開日 | 2006-11-24 |
| DOI | 10.1074/jbc.M606383200 |
| PII | S0021-9258(20)68139-X |
| PMID | 16982622 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry* Arabidopsis Proteins / genetics* Carbon / chemistry Catalysis Enoyl-CoA Hydratase / chemistry* Enoyl-CoA Hydratase / genetics Fatty Acids / chemistry Fatty Acids, Unsaturated / chemistry* Molecular Sequence Data Onions Oxygen / chemistry Peroxisomes / chemistry Peroxisomes / enzymology* Plant Proteins / chemistry Recombinant Fusion Proteins / chemistry Saccharomyces cerevisiae / metabolism Sequence Homology, Amino Acid |
| IF | 4.238 |
| 引用数 | 20 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda03253 |