RRC ID 18891
Author Imazato S, Horikawa D, Takeda K, Kiba W, Izutani N, Yoshikawa R, Hayashi M, Ebisu S, Nakano T.
Title Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials.
Journal Dent Mater J
Abstract This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.
Volume 29(3)
Pages 341-6
Published 2010-5
PII JST.JSTAGE/dmj/2009-082
PMID 20467157
MeSH Alkaline Phosphatase / biosynthesis Animals Cell Adhesion Cell Differentiation Cell Line Cell Proliferation Composite Resins* Dental Restoration, Permanent Glass Ionomer Cements* Hydroxybenzoate Ethers Hydroxybenzoates Mesenchymal Stromal Cells / cytology* Mice Mice, Inbred C3H Pluripotent Stem Cells / cytology* Regeneration Resin Cements* Tetrazolium Salts / metabolism Thiazoles / metabolism Tissue Scaffolds* Zinc Oxide-Eugenol Cement / chemistry
IF 1.205
Times Cited 8
Human and Animal Cells C2C12 (RCB0987)