| RRC ID |
18891
|
| Author |
Imazato S, Horikawa D, Takeda K, Kiba W, Izutani N, Yoshikawa R, Hayashi M, Ebisu S, Nakano T.
|
| Title |
Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials.
|
| Journal |
Dent Mater J
|
| Abstract |
This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.
|
| Volume |
29(3)
|
| Pages |
341-6
|
| Published |
2010-5-1
|
| DOI |
10.4012/dmj.2009-082
|
| PII |
JST.JSTAGE/dmj/2009-082
|
| PMID |
20467157
|
| MeSH |
Alkaline Phosphatase / biosynthesis
Animals
Cell Adhesion
Cell Differentiation
Cell Line
Cell Proliferation
Composite Resins*
Dental Restoration, Permanent
Glass Ionomer Cements*
Hydroxybenzoate Ethers
Hydroxybenzoates
Mesenchymal Stem Cells / cytology*
Mice
Mice, Inbred C3H
Pluripotent Stem Cells / cytology*
Regeneration
Resin Cements*
Tetrazolium Salts / metabolism
Thiazoles / metabolism
Tissue Scaffolds*
Zinc Oxide-Eugenol Cement / chemistry
|
| IF |
1.359
|
| Times Cited |
15
|
|
WOS Category
|
DENTISTRY, ORAL SURGERY & MEDICINE
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
