Reference - Detail
| RRC ID | 18894 |
|---|---|
| Author | Xiao L, Aoshima H, Saitoh Y, Miwa N. |
| Title | The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system. |
| Journal | Biomaterials |
| Abstract |
Effects of squalane-dissolved fullerene-C60 (Sql-fullerene) on macrophage activation and adipose conversion with oxidative stress were studied using an inflammatory adipose-tissue equivalent (ATE) and OP9 mouse stromal preadipocyte-U937 lymphoma cell co-culture systems. Differentiation of OP9 cells was initiated by insulin-rich serum replacement (SR) as an adipogenic stimulant, and then followed by accumulation of intracellular lipid droplets and reactive oxygen species (ROS), both of which were significantly inhibited by Sql-fullerene. In the OP9-U937 cell co-culture system, U937 cells rapidly differentiated to macrophage-like cells during SR-induced adipogenesis in OP9 cells. The ROS accumulation was in the co-culture more marked than in OP9 cells alone, suggesting that the interaction between adipocytes and monocytes/macrophages promotes inflammatory responses. Sql-fullerene significantly inhibited macrophage activation and low-grade adipogenesis in the OP9-U937 co-culture system. We developed a three-dimensional inflammatory adipose-tissue model "ATE" consisting of, characteristically, U937 cells in the culture-wells, and, in addition, mounted a culture insert containing OP9 cells-populated collagen gel. ATE is enabled with suitable stimulation to represent the pathology of inflammatory disorders, such as macrophage infiltration in adipose tissue. Five-day culturing of ATE in SR medium occurred U937 macrophage migration and intracellular oil-droplet accumulation that were significantly inhibited by Sql-fullerene. Our results suggest that Sql-fullerene might be explored as a potential medicine for the treatment of metabolic syndrome or other obesity-related disorders. |
| Volume | 31(23) |
| Pages | 5976-85 |
| Published | 2010-8-1 |
| DOI | 10.1016/j.biomaterials.2010.04.032 |
| PII | S0142-9612(10)00548-X |
| PMID | 20488530 |
| MeSH | Adipocytes / metabolism Adipogenesis* Animals Cell Differentiation Cell Line Cell Line, Tumor Coculture Techniques Fullerenes / chemistry* Humans Macrophage Activation* Mice Microscopy, Electron, Scanning Oxidative Stress* Reactive Oxygen Species / metabolism Squalene / chemistry* |
| IF | 10.317 |
| Times Cited | 24 |
| WOS Category | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Podcasts |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9(RCB1124) |