論文 - 詳細
| RRC ID | 1890 |
|---|---|
| 著者 | Nour-Eldin HH, Hansen BG, Nørholm MH, Jensen JK, Halkier BA. |
| タイトル | Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy. The original features of the technique, namely simplicity, speed, high efficiency and low cost are thus combined with high fidelity as well as a transparent, simple and flexible vector design. A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed. |
| 巻・号 | 34(18) |
| ページ | e122 |
| 公開日 | 2006-1-1 |
| DOI | 10.1093/nar/gkl635 |
| PII | gkl635 |
| PMID | 17000637 |
| PMC | PMC1635280 |
| MeSH | Cloning, Molecular / methods* DNA-Directed DNA Polymerase / metabolism Deoxyribonucleases, Type II Site-Specific Genetic Vectors Polymerase Chain Reaction* Uracil / metabolism* |
| IF | 11.502 |
| 引用数 | 297 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda05545 pda03530 pda01560 pda04389 pda04574 |