| RRC ID |
1890
|
| 著者 |
Nour-Eldin HH, Hansen BG, Nørholm MH, Jensen JK, Halkier BA.
|
| タイトル |
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern cloning techniques. Its application has, however, been hampered by incompatibility with proof-reading DNA polymerases. We have advanced the technique by identifying PfuCx as a compatible proof-reading DNA polymerase and by developing an improved vector design strategy. The original features of the technique, namely simplicity, speed, high efficiency and low cost are thus combined with high fidelity as well as a transparent, simple and flexible vector design. A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed.
|
| 巻・号 |
34(18)
|
| ページ |
e122
|
| 公開日 |
2006-1-1
|
| DOI |
10.1093/nar/gkl635
|
| PII |
gkl635
|
| PMID |
17000637
|
| PMC |
PMC1635280
|
| MeSH |
Cloning, Molecular / methods*
DNA-Directed DNA Polymerase / metabolism
Deoxyribonucleases, Type II Site-Specific
Genetic Vectors
Polymerase Chain Reaction*
Uracil / metabolism*
|
| IF |
11.502
|
| 引用数 |
297
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05545
pda03530
pda01560
pda04389
pda04574 |
