論文 - 詳細
| RRC ID | 18914 |
|---|---|
| 著者 | Akman AC, Seda Tiğli R, Gümüşderelioğlu M, Nohutcu RM. |
| タイトル | Bone morphogenetic protein-6-loaded chitosan scaffolds enhance the osteoblastic characteristics of MC3T3-E1 cells. |
| ジャーナル | Artif Organs |
| Abstract |
The purpose of this study is to investigate the convenience of bone morphogenetic protein-6 (BMP-6)-loaded chitosan scaffolds with preosteoblastic cells for bone tissue engineering. MC3T3-E1 cells were seeded into three different groups: chitosan scaffolds, BMP-6-loaded chitosan scaffolds, and chitosan scaffolds with free BMP-6 in culture medium. Tissue-engineered constructs were characterized by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay, scanning electron microscopy (SEM), mineralization assay (von Kossa), alkaline phosphatase (ALP) activity, and osteocalcin (OCN) assays. BMP-6-loaded chitosan scaffolds supported proliferation of the MC3T3-E1 mouse osteogenic cells in a similar pattern as the unloaded chitosan scaffolds group and as the chitosan scaffolds with free BMP-6 group. SEM images of the cell-seeded scaffolds revealed significant acceleration of extracellular matrix synthesis in BMP-6-loaded chitosan scaffolds. Both levels of ALP and OCN were higher in BMP-6-loaded chitosan scaffold group compared with the other two groups. In addition, BMP-6-loaded scaffolds showed strong staining in mineralization assays. These findings suggest that BMP-6-loaded chitosan scaffold supports cellular functions of the osteoblastic cells; therefore, this scaffold is considered as a new promising vehicle for bone tissue engineering applications. |
| 巻・号 | 34(1) |
| ページ | 65-74 |
| 公開日 | 2010-1-1 |
| DOI | 10.1111/j.1525-1594.2009.00798.x |
| PII | AOR798 |
| PMID | 19821811 |
| MeSH | Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 6 / physiology* Bone and Bones / physiology* Bone and Bones / ultrastructure Cell Differentiation Cell Line Cell Proliferation Chitosan Humans Mice Osteoblasts / physiology* Osteocalcin / metabolism Tissue Engineering* Tissue Scaffolds* |
| IF | 2.259 |
| 引用数 | 31 |
| WOS 分野 | TRANSPLANTATION ENGINEERING, BIOMEDICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |