RRC ID 18939
著者 Iacono A, Gómez R, Sperry J, Conde J, Bianco G, Meli R, Gómez-Reino JJ, Smith AB 3rd, Gualillo O.
タイトル Effect of oleocanthal and its derivatives on inflammatory response induced by lipopolysaccharide in a murine chondrocyte cell line.
ジャーナル Arthritis Rheum
Abstract OBJECTIVE:In joint diseases, cartilage homeostasis is disrupted by mechanisms that are driven by combinations of biologic factors that vary according to the disease process. In osteoarthritis (OA), biomechanical stimuli predominate, with up-regulation of both catabolic and anabolic factors. Likewise, OA progression is characterized by increased nitric oxide (NO) production, which has been associated with cartilage degradation. Given the relevance of cartilage degenerative diseases in our society, the development of a novel pharmacologic intervention is a critically important public health goal. Recently, oleocanthal isolated from extra virgin olive oil was found to display nonsteroidal antiinflammatory drug activity similar to that of ibuprofen, a drug widely used in the therapeutic management of joint inflammatory diseases. We undertook this study to evaluate the effect of oleocanthal and its derivatives on the modulation of NO production in chondrocytes.
METHODS:Cultured ATDC-5 chondrocytes were tested with different doses of oleocanthal and its derivatives. Cell viability was evaluated using the MTT assay. Nitrite accumulation was determined in culture supernatant using the Griess reaction. Inducible NO synthase (NOS2) protein expression was examined using Western blotting analysis.
RESULTS:Oleocanthal and its derivatives decreased lipopolysaccharide-induced NOS2 synthesis in chondrocytes without significantly affecting cell viability at lower concentrations. Among the derivatives we examined, derivative 231 was the most interesting, since its inhibitory effect on NOS2 was devoid of cytotoxicity even at higher concentrations.
CONCLUSION:This class of molecules shows potential as a therapeutic weapon for the treatment of inflammatory degenerative joint diseases.
巻・号 62(6)
ページ 1675-82
公開日 2010-6-1
DOI 10.1002/art.27437
PMID 20201078
MeSH Aldehydes / pharmacology* Analysis of Variance Animals Blotting, Western Cell Line Cell Survival / drug effects Cells, Cultured Chondrocytes / drug effects* Chondrocytes / metabolism* Cyclopentane Monoterpenes Dose-Response Relationship, Drug Inflammation / chemically induced Inflammation / drug therapy* Lipopolysaccharides / pharmacology Mice Nitric Oxide Synthase Type II / metabolism Phenols / pharmacology*
引用数 65
WOS 分野 RHEUMATOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)