RRC ID 18947
著者 Tanae K, Horiuchi T, Yamakawa T, Matsuo Y, Kawamukai M.
タイトル Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast.
ジャーナル FEBS Lett
Abstract An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.
巻・号 586(23)
ページ 4190-6
公開日 2012-11-30
DOI 10.1016/j.febslet.2012.10.020
PII S0014-5793(12)00803-4
PMID 23089178
MeSH Chromatin / drug effects Chromatin / metabolism Hydroxyurea / pharmacology Molecular Chaperones / genetics Molecular Chaperones / metabolism* Nuclear Proteins / genetics Nuclear Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Thiabendazole / pharmacology
IF 3.057
引用数 4
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
酵母 FY21608(SKP605-33) FY21609(SKP593-33P) FY21594(SKP561-15) FY21615(SKP593-34) FY21618(KT166)