論文 - 詳細
RRC ID | 18947 |
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著者 | Tanae K, Horiuchi T, Yamakawa T, Matsuo Y, Kawamukai M. |
タイトル | Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast. |
ジャーナル | FEBS Lett |
Abstract |
An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1-33 and asf1-30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1-33 mutants at high temperatures. Furthermore, asf1-33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1-33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone. |
巻・号 | 586(23) |
ページ | 4190-6 |
公開日 | 2012-11-30 |
DOI | 10.1016/j.febslet.2012.10.020 |
PII | S0014-5793(12)00803-4 |
PMID | 23089178 |
MeSH | Chromatin / drug effects Chromatin / metabolism Hydroxyurea / pharmacology Molecular Chaperones / genetics Molecular Chaperones / metabolism* Nuclear Proteins / genetics Nuclear Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Thiabendazole / pharmacology |
IF | 3.057 |
引用数 | 4 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
酵母 | FY21608(SKP605-33) FY21609(SKP593-33P) FY21594(SKP561-15) FY21615(SKP593-34) FY21618(KT166) |