RRC ID 1897
Author Limbourg A, Ploom M, Elligsen D, Sörensen I, Ziegelhoeffer T, Gossler A, Drexler H, Limbourg FP.
Title Notch ligand Delta-like 1 is essential for postnatal arteriogenesis.
Journal Circ. Res.
Abstract Growth of functional arteries is essential for the restoration of blood flow to ischemic organs. Notch signaling regulates arterial differentiation upstream of ephrin-B2 during embryonic development, but its role during postnatal arteriogenesis is unknown. Here, we identify the Notch ligand Delta-like 1 (Dll1) as an essential regulator of postnatal arteriogenesis. Dll1 expression was specifically detected in arterial endothelial cells, but not in venous endothelial cells or capillaries. During ischemia-induced arteriogenesis endothelial Dll1 expression was strongly induced, Notch signaling activated and ephrin-B2 upregulated, whereas perivascular cells expressed proangiogenic vascular endothelial growth factor, and the ephrin-B2 activator EphB4. In heterozygous Dll1 mutant mice endothelial Notch activation and ephrin-B2 induction after hindlimb ischemia were absent, arterial collateral growth was abrogated and recovery of blood flow was severely impaired, but perivascular vascular endothelial growth factor and EphB4 expression was unaltered. In vitro, angiogenic growth factors synergistically activated Notch signaling by induction of Dll1, which was necessary and sufficient to regulate ephrin-B2 expression and to induce ephrin-B2 and EphB4-dependent branching morphogenesis in human arterial EC. Thus, Dll1-mediated Notch activation regulates ephrin-B2 expression and postnatal arteriogenesis.
Volume 100(3)
Pages 363-71
Published 2007-2-16
DOI 10.1161/01.RES.0000258174.77370.2c
PII 01.RES.0000258174.77370.2c
PMID 17234965
MeSH Animals Aorta / cytology Arteries / chemistry Arteries / cytology* Arteries / growth & development Capillaries / chemistry Cells, Cultured / drug effects Cells, Cultured / metabolism Collateral Circulation / physiology Constriction Culture Media, Serum-Free Endothelial Cells / metabolism Endothelium, Vascular / cytology* Gene Expression Regulation / physiology* Gene Silencing Hindlimb / blood supply Humans Intercellular Signaling Peptides and Proteins / deficiency Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / physiology* Ischemia / etiology Ischemia / genetics Ischemia / physiopathology* Membrane Proteins / physiology* Mice Mice, Transgenic Morphogenesis / genetics Morphogenesis / physiology Neovascularization, Physiologic / genetics Neovascularization, Physiologic / physiology* Organ Specificity RNA, Small Interfering / pharmacology Receptor, EphB2 / biosynthesis Receptor, EphB2 / genetics Receptor, EphB2 / physiology Receptor, EphB4 / biosynthesis Receptor, EphB4 / genetics Receptor, EphB4 / physiology Receptors, Notch / physiology* Veins / chemistry
IF 15.211
Times Cited 86
WOS Category CARDIAC & CARDIOVASCULAR SYSTEMS PERIPHERAL VASCULAR DISEASE HEMATOLOGY
Resource
DNA material pCMX-N/RBP-J (R218H) (RDB03022)