論文 - 詳細
| RRC ID | 18971 |
|---|---|
| 著者 | Khuda II, Koide N, Noman AS, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T. |
| タイトル | Seladin-1 is a novel lipopolysaccharide (LPS)-responsive gene and inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS. |
| ジャーナル | Immunology |
| Abstract |
Selective Alzheimer disease indicator-1 (seladin-1) is a broadly expressed oxidoreductase and is related to Alzheimer disease, cholesterol metabolism and carcinogenesis. The effect of lipopolysaccharide (LPS) on the expression of seladin-1 was examined using RAW 264.7 macrophage-like cells and murine peritoneal macrophages. Lipopolysaccharide induced the expression of seladin-1 protein and messenger RNA in those macrophages. The seladin-1 expression was also augmented by a series of Toll-like receptor ligands. The LPS augmented the expression of seladin-1 via reactive oxygen species generation and p38 activation. Seladin-1 inhibited LPS-induced activation of p38 but not nuclear factor-kappaB and inhibited the production of tumour necrosis factor-alpha in response to LPS. Moreover, seladin-1 inhibited LPS-induced osteoclast formation and enhanced LPS-induced alkaline phosphatase activity. Therefore, it was suggested that seladin-1 might be an LPS-responsible gene product and regulate the LPS-induced inflammatory response negatively. |
| 巻・号 | 131(1) |
| ページ | 59-66 |
| 公開日 | 2010-9-1 |
| DOI | 10.1111/j.1365-2567.2010.03274.x |
| PII | IMM3274 |
| PMID | 20406300 |
| PMC | PMC2966758 |
| MeSH | Animals Cell Line Lipopolysaccharides / immunology Lipopolysaccharides / pharmacology* Macrophages / drug effects* Macrophages / immunology Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / immunology Macrophages, Peritoneal / metabolism Mice Nerve Tissue Proteins / drug effects Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Osteoclasts / cytology Osteoclasts / metabolism Oxidoreductases Acting on CH-CH Group Donors / drug effects Oxidoreductases Acting on CH-CH Group Donors / genetics Oxidoreductases Acting on CH-CH Group Donors / metabolism* Polymerase Chain Reaction Tumor Necrosis Factor-alpha / antagonists & inhibitors* Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / drug effects |
| IF | 5.016 |
| 引用数 | 3 |
| WOS 分野 | IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |