| RRC ID |
18977
|
| 著者 |
Gushima M, Kawate H, Ohnaka K, Nomura M, Takayanagi R.
|
| タイトル |
Raloxifene induces nucleolar translocation of the estrogen receptor.
|
| ジャーナル |
Mol Cell Endocrinol
|
| Abstract |
Raloxifene (RLX), a selective estrogen receptor modulator (SERM), binds to the estrogen receptor alpha (ERalpha) and acts as an agonist in some tissues, and as an antagonist in others. To clarify the molecular mechanism underlying the tissue specificity of SERMs, we examined the intracellular localization of ERalpha using a green fluorescent protein (GFP)-tagged protein in culture cells from various tissues. Although ERalpha formed intranuclear foci in the presence of estradiol (E(2)), RLX translocated ERalpha into the nucleoli in breast cancer cell lines. This phenomenon was not observed in cells from other tissues. Immunofluorescence staining revealed that endogenous ERalpha was also translocated into the nucleoli in the presence of RLX. Mutation analyses demonstrate that helix 12 of ERalpha is essential to the nucleolar translocation of ERalpha. These results suggest that translocation of ERalpha into the nucleoli is RLX-specific and is a key event for RLX-induced growth repression of mammary gland cells.
|
| 巻・号 |
319(1-2)
|
| ページ |
14-22
|
| 公開日 |
2010-5-5
|
| DOI |
10.1016/j.mce.2010.01.006
|
| PII |
S0303-7207(10)00008-0
|
| PMID |
20083158
|
| MeSH |
Animals
Cell Nucleolus / metabolism*
Cells, Cultured
Estrogen Receptor alpha / metabolism*
Fluorescence Recovery After Photobleaching
Fluorescent Antibody Technique
Green Fluorescent Proteins / metabolism
Humans
Microscopy, Confocal
Protein Structure, Secondary / physiology
Protein Transport / drug effects
Raloxifene Hydrochloride / pharmacology*
Selective Estrogen Receptor Modulators / pharmacology
|
| IF |
3.871
|
| 引用数 |
7
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
Saos-2(RCB0428) |
