論文 - 詳細
RRC ID | 18984 |
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著者 | Tsukamoto A, Hayashida Y, Furukawa KS, Ushida T. |
タイトル | Spatio-temporal PLC activation in parallel with intracellular Ca2+ wave propagation in mechanically stimulated single MDCK cells. |
ジャーナル | Cell Calcium |
Abstract |
Intracellular Ca2+ transients are evoked either by the opening of Ca2+ channels on the plasma membrane or by phospholipase C (PLC) activation resulting in IP3 production. Ca2+ wave propagation is known to occur in mechanically stimulated cells; however, it remains uncertain whether and how PLC activation is involved in intracellular Ca2+ wave propagation in mechanically stimulated cells. To answer these questions, it is indispensable to clarify the spatio-temporal relations between intracellular Ca2+ wave propagation and PLC activation. Thus, we visualized both cytosolic Ca2+ and PLC activation using a real-time dual-imaging system in individual Mardin-Darby Canine Kidney (MDCK) cells. This system allowed us to simultaneously observe intracellular Ca2+ wave propagation and PLC activation in a spatio-temporal manner in a single mechanically stimulated MDCK cell. The results showed that PLC was activated not only in the mechanically stimulated region but also in other subcellular regions in parallel with intracellular Ca2+ wave propagation. These results support a model in which PLC is involved in Ca2+ signaling amplification in mechanically stimulated cells. |
巻・号 | 47(3) |
ページ | 253-63 |
公開日 | 2010-3-1 |
DOI | 10.1016/j.ceca.2009.12.008 |
PII | S0143-4160(09)00202-4 |
PMID | 20060585 |
MeSH | Animals Calcium / metabolism* Calcium Signaling / physiology* Cell Line Cell Membrane / metabolism* Cytosol / metabolism Dogs Enzyme Activation / physiology Fluorescent Dyes Image Processing, Computer-Assisted / methods Inositol Phosphates / metabolism Intracellular Fluid / metabolism Microscopy, Fluorescence / methods Physical Stimulation Signal Transduction / physiology* Stress, Mechanical Type C Phospholipases / metabolism* |
IF | 4.874 |
引用数 | 5 |
WOS 分野 | CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | MDCK(RCB0995) |