RRC ID 18984
著者 Tsukamoto A, Hayashida Y, Furukawa KS, Ushida T.
タイトル Spatio-temporal PLC activation in parallel with intracellular Ca2+ wave propagation in mechanically stimulated single MDCK cells.
ジャーナル Cell Calcium
Abstract Intracellular Ca2+ transients are evoked either by the opening of Ca2+ channels on the plasma membrane or by phospholipase C (PLC) activation resulting in IP3 production. Ca2+ wave propagation is known to occur in mechanically stimulated cells; however, it remains uncertain whether and how PLC activation is involved in intracellular Ca2+ wave propagation in mechanically stimulated cells. To answer these questions, it is indispensable to clarify the spatio-temporal relations between intracellular Ca2+ wave propagation and PLC activation. Thus, we visualized both cytosolic Ca2+ and PLC activation using a real-time dual-imaging system in individual Mardin-Darby Canine Kidney (MDCK) cells. This system allowed us to simultaneously observe intracellular Ca2+ wave propagation and PLC activation in a spatio-temporal manner in a single mechanically stimulated MDCK cell. The results showed that PLC was activated not only in the mechanically stimulated region but also in other subcellular regions in parallel with intracellular Ca2+ wave propagation. These results support a model in which PLC is involved in Ca2+ signaling amplification in mechanically stimulated cells.
巻・号 47(3)
ページ 253-63
公開日 2010-3-1
DOI 10.1016/j.ceca.2009.12.008
PII S0143-4160(09)00202-4
PMID 20060585
MeSH Animals Calcium / metabolism* Calcium Signaling / physiology* Cell Line Cell Membrane / metabolism* Cytosol / metabolism Dogs Enzyme Activation / physiology Fluorescent Dyes Image Processing, Computer-Assisted / methods Inositol Phosphates / metabolism Intracellular Fluid / metabolism Microscopy, Fluorescence / methods Physical Stimulation Signal Transduction / physiology* Stress, Mechanical Type C Phospholipases / metabolism*
IF 4.874
引用数 5
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 MDCK(RCB0995)