RRC ID 19015
著者 Morotomi M, Nagai F, Watanabe Y.
タイトル Description of Christensenella minuta gen. nov., sp. nov., isolated from human faeces, which forms a distinct branch in the order Clostridiales, and proposal of Christensenellaceae fam. nov.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel, strictly anaerobic, non-motile, non-spore-forming, Gram-negative, short, straight rod with tapered ends, designated YIT 12065(T), was isolated from human faeces. Strain YIT 12065(T) was saccharolytic and negative for catalase, oxidase and urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. The end products of glucose fermentation were acetic acid and a small amount of butyric acid. The DNA G+C content was 51.3 mol%. The predominant fatty acids were iso-C(15:0), C(16:0) and C(14:0). Respiratory quinones were not detected. The cell wall contained glutamic acid, serine, alanine and ll-diaminopimelic acid. The whole-cell sugars were ribose, rhamnose, galactose and glucose. Phylogenetic analyses based on 16S rRNA gene sequences using three treeing algorithms revealed that the strain formed a novel family-level lineage within the phylum Firmicutes, class Clostridia, order Clostridiales. Caldicoprobacter oshimai JW/HY-331(T) was shown to be the closest named relative on the basis of 16S rRNA gene sequence similarity (86.9%), followed by Tindallia californiensis DSM 14871(T) (86.3%) and Clostridium ganghwense JCM 13193(T) (86.1%). Similar 16S rRNA gene sequences (98.6-96.7%) were found amongst faecal uncultured clones of human and dugong (Dugong dugon). They clustered with strain YIT 12065(T) in a distinct and deep evolutionary lineage of descent in the order Clostridiales. The distinct phylogenetic position supports the proposal of Christensenella gen. nov., with the type species Christensenella minuta sp. nov. (type strain YIT 12065(T) =DSM 22607(T) =JCM 16072(T)). A new family Christensenellaceae fam. nov. is also proposed.
巻・号 62(Pt 1)
ページ 144-149
公開日 2012-1-1
DOI 10.1099/ijs.0.026989-0
PMID 21357455
MeSH Acetic Acid / metabolism Anaerobiosis Bacterial Typing Techniques Base Composition Butyrates / metabolism Carbohydrates / analysis Catalase / metabolism Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / metabolism Feces / microbiology* Fermentation Glucose / metabolism Gram-Positive Bacteria / classification* Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Humans Molecular Sequence Data Oxidoreductases / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sucrose / metabolism Urease / metabolism
IF 2.415
引用数 78
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 13193 JCM 16072