RRC ID 1905
Author Omoteyama K, Ikeda H, Imaki J, Sakai M.
Title Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors.
Journal Biochem Biophys Res Commun
Abstract The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-beta via the SMAD pathway; however, the c-Maf could not induce TGF-beta, nor could TGF-beta induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-beta independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development.
Volume 339(4)
Pages 1089-97
Published 2006-1-27
DOI 10.1016/j.bbrc.2005.11.119
PII S0006-291X(05)02647-1
PMID 16343439
MeSH Animals Cell Differentiation Chondrocytes / cytology* Chondrocytes / metabolism* Connective Tissue Growth Factor Fibroblasts / cytology* Fibroblasts / metabolism* Gene Expression Regulation / physiology Immediate-Early Proteins / metabolism* Intercellular Signaling Peptides and Proteins / metabolism* Mice Mice, Inbred C3H Promoter Regions, Genetic Proto-Oncogene Proteins c-maf / metabolism* Transcription Factors / metabolism Transcriptional Activation / physiology
IF 2.985
Times Cited 11
DNA material AxCALNLrmaf2 (RDB02410) AxCANCre (RDB01748).