論文 - 詳細
| RRC ID | 1905 |
|---|---|
| 著者 | Omoteyama K, Ikeda H, Imaki J, Sakai M. |
| タイトル | Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-beta via the SMAD pathway; however, the c-Maf could not induce TGF-beta, nor could TGF-beta induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-beta independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development. |
| 巻・号 | 339(4) |
| ページ | 1089-97 |
| 公開日 | 2006-1-27 |
| DOI | 10.1016/j.bbrc.2005.11.119 |
| PII | S0006-291X(05)02647-1 |
| PMID | 16343439 |
| MeSH | Animals Cell Differentiation Chondrocytes / cytology* Chondrocytes / metabolism* Connective Tissue Growth Factor Fibroblasts / cytology* Fibroblasts / metabolism* Gene Expression Regulation / physiology Immediate-Early Proteins / metabolism* Intercellular Signaling Peptides and Proteins / metabolism* Mice Mice, Inbred C3H Promoter Regions, Genetic Proto-Oncogene Proteins c-maf / metabolism* Transcription Factors / metabolism Transcriptional Activation / physiology |
| IF | 2.985 |
| 引用数 | 11 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCALNLrmaf2 (RDB02410) AxCANCre (RDB01748). |