RRC ID 19063
Author Boden R, Cleland D, Green PN, Katayama Y, Uchino Y, Murrell JC, Kelly DP.
Title Phylogenetic assessment of culture collection strains of Thiobacillus thioparus, and definitive 16S rRNA gene sequences for T. thioparus, T. denitrificans, and Halothiobacillus neapolitanus.
Journal Arch Microbiol
Abstract The 16S rRNA gene sequences of 12 strains of Thiobacillus thioparus held by different culture collections have been compared. A definitive sequence for the reference type strain (Starkey; ATCC 8158(T)) was obtained. The sequences for four examples of the Starkey type strain were essentially identical, confirming their sustained identity after passage through different laboratories. One strain (NCIMB 8454) was reassigned as a strain of Halothiobacillus neapolitanus, and a second (NCIMB 8349) was a species of Thermithiobacillus. These two strains have been renamed in their catalog by the National Collection of Industrial and Marine Bacteria. The 16S rRNA gene sequence of the type strain of Halothiobacillus neapolitanus (NCIMB 8539(T)) was determined and used to confirm the identity of other culture collection strains of this species. The reference sequences for the type strains of Thiobacillus thioparus and Halothiobacillus neapolitanus have been added to the online List of Prokaryotic Names with Standing in Nomenclature. Comparison of the 16S rRNA gene sequences available for strains of Thiobacillus denitrificans indicated that the sequence for the type strain (NCIMB 9548(T)) should always be used as the reference sequence for new and existing isolates.
Volume 194(3)
Pages 187-95
Published 2012-3-1
DOI 10.1007/s00203-011-0747-0
PMID 21858648
MeSH Genes, rRNA Halothiobacillus / classification* Halothiobacillus / genetics Molecular Sequence Data Phylogeny* RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics* Sequence Analysis, RNA Thiobacillus / classification* Thiobacillus / genetics
IF 1.884
Times Cited 15
WOS Category MICROBIOLOGY
Resource
General Microbes JCM 3859