| RRC ID |
19066
|
| Author |
Puramattathu TV, Islam MR, Nishie M, Yanagihara S, Nagao J, Okuda K, Zendo T, Nakayama J, Sonomoto K.
|
| Title |
Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes.
|
| Journal |
Appl Microbiol Biotechnol
|
| Abstract |
Nukacin D13E (D13E) is a variant of type-A(II) lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. D13E exhibited a twofold higher specific antimicrobial activity than nukacin ISK-1 against a number of Gram-positive bacteria. We previously reported the heterologous production of D13E in Lactococcus lactis NZ9000 under the control of nisin-controlled gene expression system. In this study, we demonstrated enhanced production of D13E by the additional expression of immunity genes, nukFEG. The nukacin ISK-1 immunity, conferred by the ABC transporter complex, NukFEG, and the lantibiotic-binding protein, NukH, was not overwhelmed by D13E. The additional NukFEG resulted in a fourfold increase in the immunity level of the strain and a 5.2-fold increase in D13E production. The additional NukFEGH-expressing strain with the highest D13E immunity showed reduced level of production. Further improvement in D13E production was achieved by using pH-controlled batch fermentation.
|
| Volume |
93(2)
|
| Pages |
671-8
|
| Published |
2012-1-1
|
| DOI |
10.1007/s00253-011-3563-1
|
| PMID |
21904816
|
| MeSH |
Bacteriocins / genetics
Bacteriocins / metabolism*
Biological Transport
Drug Resistance, Bacterial*
Gene Expression
Lactococcus lactis / genetics
Lactococcus lactis / metabolism*
Membrane Transport Proteins / genetics
Membrane Transport Proteins / metabolism
Mutant Proteins / genetics
Mutant Proteins / metabolism
Mutation, Missense
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
|
| IF |
3.53
|
| Times Cited |
3
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 1157
JCM 11004 |
