論文 - 詳細
| RRC ID | 19135 |
|---|---|
| 著者 | Morita T, Takegawa K. |
| タイトル | A simple and efficient procedure for transformation of Schizosaccharomyces pombe. |
| ジャーナル | Yeast |
| Abstract |
We describe a simple and efficient procedure for transformation of Schizosaccharomyces pombe. Sz. pombe colonies grown on minimal (SD) plates were directly removed and suspended in a 100 microl reaction mixture containing 70 microl PLATE solution (50% polyethylene glycol-4000, 100 mM lithium acetate, 10 mM Tris-HCl, pH 4.9, and 1 mM EDTA), 10 microl plasmid DNA (1 microg), 10 microl carrier DNA (100 microg) and 10 microl sterile distilled water. After incubation at 30 degrees C for 1 h followed by heat shock treatment at 42 degrees C for 15 min, the reaction mixture was spread on a selection plate. The transformation efficiency obtained using the procedure was approximately 8000 transformants/microg DNA. The method is simple and time-saving, making it especially useful for a large number of samples and when a high transformation efficiency is not required. |
| 巻・号 | 21(8) |
| ページ | 613-7 |
| 公開日 | 2004-6-1 |
| DOI | 10.1002/yea.1104 |
| PMID | 15197727 |
| MeSH | Plasmids / genetics Polyethylene Glycols / metabolism Schizosaccharomyces / genetics* Transduction, Genetic / methods* Transformation, Bacterial / physiology* Transformation, Genetic / physiology* |
| IF | 3.143 |
| 引用数 | 53 |
| WOS 分野 | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FYP4216(pAL-SK) FYP4207(pREP1) |