RRC ID 19135
著者 Morita T, Takegawa K.
タイトル A simple and efficient procedure for transformation of Schizosaccharomyces pombe.
ジャーナル Yeast
Abstract We describe a simple and efficient procedure for transformation of Schizosaccharomyces pombe. Sz. pombe colonies grown on minimal (SD) plates were directly removed and suspended in a 100 microl reaction mixture containing 70 microl PLATE solution (50% polyethylene glycol-4000, 100 mM lithium acetate, 10 mM Tris-HCl, pH 4.9, and 1 mM EDTA), 10 microl plasmid DNA (1 microg), 10 microl carrier DNA (100 microg) and 10 microl sterile distilled water. After incubation at 30 degrees C for 1 h followed by heat shock treatment at 42 degrees C for 15 min, the reaction mixture was spread on a selection plate. The transformation efficiency obtained using the procedure was approximately 8000 transformants/microg DNA. The method is simple and time-saving, making it especially useful for a large number of samples and when a high transformation efficiency is not required.
巻・号 21(8)
ページ 613-7
公開日 2004-6-1
DOI 10.1002/yea.1104
PMID 15197727
MeSH Plasmids / genetics Polyethylene Glycols / metabolism Schizosaccharomyces / genetics* Transduction, Genetic / methods* Transformation, Bacterial / physiology* Transformation, Genetic / physiology*
IF 3.143
引用数 53
WOS 分野 MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FYP4216(pAL-SK) FYP4207(pREP1)