RRC ID 19141
著者 Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
タイトル Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions.
ジャーナル Cell Rep
Abstract After implantation, pluripotent epiblasts are converted to embryonic ectoderm through cell-cell interactions that significantly change the transcriptional and epigenetic networks. An entrée to understanding this vital developmental transition is the t(w5) mutation of the mouse t complex. This mutation produces highly specific defects in the embryonic ectoderm before gastrulation, leading to death of the embryonic ectoderm. Using a positional cloning approach, we have now identified the mutated gene, completing a decades-long search. The gene, vacuolar protein sorting 52 (Vps52), is a mouse homolog of yeast VPS52 that is involved in the retrograde trafficking of endosomes. Our data suggest that Vps52 acts in extraembryonic tissues to support the growth and differentiation of embryonic ectoderm via cell-cell interactions. It is also required in the formation of embryonic structures at a later stage of development, revealing hitherto unknown functions of Vps52 in the development of a multicellular organism.
巻・号 2(5)
ページ 1363-74
公開日 2012-11-29
DOI 10.1016/j.celrep.2012.10.004
PII S2211-1247(12)00340-3
PMID 23142660
MeSH Animals Base Sequence Cell Communication Cell Differentiation Cells, Cultured Ectoderm / metabolism Ectoderm / pathology Embryo, Mammalian / metabolism Embryo, Mammalian / pathology Embryonic Development Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Exons Frameshift Mutation Haplotypes Major Histocompatibility Complex / genetics Mice Mice, Knockout Mice, Transgenic Mitosis Molecular Sequence Data Proteins / genetics Proteins / metabolism* Vesicular Transport Proteins t-Complex Genome Region*
IF 8.109
引用数 21
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 EB3(AES0139)
実験動物マウス RBRC00821 RBRC01828