論文 - 詳細
| RRC ID | 19148 |
|---|---|
| 著者 | Wada H, Kojo S, Kusama C, Okamoto N, Sato Y, Ishizuka B, Seino K. |
| タイトル | Successful differentiation to T cells, but unsuccessful B-cell generation, from B-cell-derived induced pluripotent stem cells. |
| ジャーナル | Int Immunol |
| Abstract |
Forced expression of certain transcription factors in somatic cells results in generation of induced pluripotent stem (iPS) cells, which differentiate into various cell types. We investigated T-cell and B-cell lineage differentiation from iPS cells in vitro. To evaluate the impact of iPS cell source, murine splenic B-cell-derived iPS (B-iPS) cells were generated after retroviral transduction of four transcription factors (Oct4, Sox2, Klf4 and c-Myc). B-iPS cells were identical to embryonic stem (ES) cells and mouse embryonic fibroblast (MEF)-derived iPS cells in morphology, ES cell marker expression as well as teratoma and chimera mouse formation. Both B-iPS and MEF-derived iPS cells differentiated into lymphocytes in OP9 co-culture systems. Both efficiently differentiated into T-cell lineage that produced IFN-γ on T-cell receptor stimulation. However, iPS cells including B-iPS cells were relatively resistant to B-cell lineage differentiation. One of the reasons of the failure of B-cell lineage differentiation seemed due to a defect of Pax5 expression in the differentiated cells. Therefore, current in vitro differentiation systems using iPS cells are sufficient for inducing T-cell but not B-cell lineage. |
| 巻・号 | 23(1) |
| ページ | 65-74 |
| 公開日 | 2011-1-1 |
| DOI | 10.1093/intimm/dxq458 |
| PII | dxq458 |
| PMID | 21135032 |
| MeSH | Animals B-Lymphocytes / cytology* B-Lymphocytes / immunology Cell Differentiation / immunology* Cell Line Cell Lineage / genetics Coculture Techniques CpG Islands DNA Methylation Gene Expression Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / immunology Kruppel-Like Factor 4 Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, SCID PAX5 Transcription Factor / genetics T-Lymphocytes / cytology* T-Lymphocytes / immunology |
| IF | 3.519 |
| 引用数 | 24 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00144 |