論文 - 詳細
| RRC ID | 19158 |
|---|---|
| 著者 | Wakabayashi T, Matsumine A, Nakazora S, Hasegawa M, Iino T, Ota H, Sonoda H, Sudo A, Uchida A. |
| タイトル | Fibulin-3 negatively regulates chondrocyte differentiation. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Fibulin-3 is a member of the fibulin family that has been newly recognized as extracellular matrix proteins. We assessed the effects of fibulin-3 overexpression on chondrocyte differentiation using the clonal murine cell line ATDC5. The ATDC5-FBLN3 stably expressing fibulin-3 protein was spindle-shaped cell compared to the ATDC5-mock with plump cell. The cell growth in the ATDC5-FBLN3 was accelerated in comparison to that in the ATDC5-mock. The ATDC5-FBLN3 was not stained by Alcian blue, nor was there any cartilage aggregate formed after the induction of chondrogenic differentiation. The expression of type II collagen, aggrecan, and type X collagen was completely suppressed in ATDC5-FBLN3 even after the induction of differentiation. The overexpression of fibulin-3 reduced the expression of Sox5 and Sox6, while it maintained the expression of Sox9. These findings suggest that fibulin-3 may play an important role as a negative regulator of chondrocyte differentiation. |
| 巻・号 | 391(1) |
| ページ | 1116-21 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/j.bbrc.2009.12.034 |
| PII | S0006-291X(09)02407-3 |
| PMID | 20005202 |
| MeSH | Animals Cartilage / cytology* Cartilage / metabolism Cell Differentiation* Cell Line, Tumor Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Humans Mice Proteoglycans / biosynthesis SOX9 Transcription Factor / metabolism SOXD Transcription Factors / metabolism |
| IF | 2.985 |
| 引用数 | 23 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |