RRC ID |
19158
|
著者 |
Wakabayashi T, Matsumine A, Nakazora S, Hasegawa M, Iino T, Ota H, Sonoda H, Sudo A, Uchida A.
|
タイトル |
Fibulin-3 negatively regulates chondrocyte differentiation.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Fibulin-3 is a member of the fibulin family that has been newly recognized as extracellular matrix proteins. We assessed the effects of fibulin-3 overexpression on chondrocyte differentiation using the clonal murine cell line ATDC5. The ATDC5-FBLN3 stably expressing fibulin-3 protein was spindle-shaped cell compared to the ATDC5-mock with plump cell. The cell growth in the ATDC5-FBLN3 was accelerated in comparison to that in the ATDC5-mock. The ATDC5-FBLN3 was not stained by Alcian blue, nor was there any cartilage aggregate formed after the induction of chondrogenic differentiation. The expression of type II collagen, aggrecan, and type X collagen was completely suppressed in ATDC5-FBLN3 even after the induction of differentiation. The overexpression of fibulin-3 reduced the expression of Sox5 and Sox6, while it maintained the expression of Sox9. These findings suggest that fibulin-3 may play an important role as a negative regulator of chondrocyte differentiation.
|
巻・号 |
391(1)
|
ページ |
1116-21
|
公開日 |
2010-1-1
|
DOI |
10.1016/j.bbrc.2009.12.034
|
PII |
S0006-291X(09)02407-3
|
PMID |
20005202
|
MeSH |
Animals
Cartilage / cytology*
Cartilage / metabolism
Cell Differentiation*
Cell Line, Tumor
Chondrocytes / cytology*
Chondrocytes / metabolism
Chondrogenesis*
Extracellular Matrix Proteins / genetics
Extracellular Matrix Proteins / metabolism*
Humans
Mice
Proteoglycans / biosynthesis
SOX9 Transcription Factor / metabolism
SOXD Transcription Factors / metabolism
|
IF |
2.985
|
引用数 |
23
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
ATDC5(RCB0565) |