RRC ID 19158
著者 Wakabayashi T, Matsumine A, Nakazora S, Hasegawa M, Iino T, Ota H, Sonoda H, Sudo A, Uchida A.
タイトル Fibulin-3 negatively regulates chondrocyte differentiation.
ジャーナル Biochem Biophys Res Commun
Abstract Fibulin-3 is a member of the fibulin family that has been newly recognized as extracellular matrix proteins. We assessed the effects of fibulin-3 overexpression on chondrocyte differentiation using the clonal murine cell line ATDC5. The ATDC5-FBLN3 stably expressing fibulin-3 protein was spindle-shaped cell compared to the ATDC5-mock with plump cell. The cell growth in the ATDC5-FBLN3 was accelerated in comparison to that in the ATDC5-mock. The ATDC5-FBLN3 was not stained by Alcian blue, nor was there any cartilage aggregate formed after the induction of chondrogenic differentiation. The expression of type II collagen, aggrecan, and type X collagen was completely suppressed in ATDC5-FBLN3 even after the induction of differentiation. The overexpression of fibulin-3 reduced the expression of Sox5 and Sox6, while it maintained the expression of Sox9. These findings suggest that fibulin-3 may play an important role as a negative regulator of chondrocyte differentiation.
巻・号 391(1)
ページ 1116-21
公開日 2010-1-1
DOI 10.1016/j.bbrc.2009.12.034
PII S0006-291X(09)02407-3
PMID 20005202
MeSH Animals Cartilage / cytology* Cartilage / metabolism Cell Differentiation* Cell Line, Tumor Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Humans Mice Proteoglycans / biosynthesis SOX9 Transcription Factor / metabolism SOXD Transcription Factors / metabolism
IF 2.985
引用数 23
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)