Reference - Detail
RRC ID | 19163 |
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Author | Zeng KW, Wang XM, Ko H, Yang HO. |
Title | Neuroprotective effect of modified Wu-Zi-Yan-Zong granule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells. |
Journal | J Ethnopharmacol |
Abstract |
AIM OF THE STUDY:To investigate the neuroprotective effect of aqueous extract of modified Wu-Zi-Yan-Zong granule (MWG), a traditional Chinese herbal medicine, against CoCl(2)-induced neurotoxicity in PC12 cells. MATERIALS AND METHODS:Cell viability assay, apoptosis rate assay, ROS detection and mitochondrial membrane potential (MMP) assay were performed. In addition, cytochrome c, caspase-3, PARP and MAPKs were also detected by Western blotting. RESULTS:MWG extract increased viability and suppresses early and middle/late stage apoptosis in a dose-dependent manner in CoCl(2)-induced PC12 cells. Moreover, MWG extract decreased the level of intracellular reactive oxygen species (ROS), increased MMP, regulated Bcl-2 family protein expression (Bcl-2 and Bcl-XL) and inhibited the release of cytochrome c from the mitochondria. In addition, MWG extract attenuated activation of caspase-3 and poly ADP-ribose polymerase (PARP) and inhibited the phosphorylation of ERK, c-Jun NH(2)-terminal kinase (JNK) and p38 MAPKs. CONCLUSIONS:MWG extract exhibited significant neuroprotective effect on PC12 cells, and this effect may be associated with the suppression of ROS generation and inhibition of mitochondria-mediated caspase and MAPK signaling pathways. |
Volume | 130(1) |
Pages | 13-8 |
Published | 2010-7-6 |
DOI | 10.1016/j.jep.2010.03.018 |
PII | S0378-8741(10)00180-7 |
PMID | 20347949 |
MeSH | Animals Apoptosis / drug effects Blotting, Western Cobalt / pharmacology* Drugs, Chinese Herbal* Membrane Potentials / drug effects Neuroprotective Agents / pharmacology* PC12 Cells Rats Reactive Oxygen Species / metabolism |
IF | 3.69 |
Times Cited | 8 |
WOS Category | PLANT SCIENCES PHARMACOLOGY & PHARMACY INTEGRATIVE & COMPLEMENTARY MEDICINE CHEMISTRY, MEDICINAL |
Resource | |
Human and Animal Cells | PC-12(RCB0009) |