RRC ID 19163
著者 Zeng KW, Wang XM, Ko H, Yang HO.
タイトル Neuroprotective effect of modified Wu-Zi-Yan-Zong granule, a traditional Chinese herbal medicine, on CoCl2-induced PC12 cells.
ジャーナル J Ethnopharmacol
Abstract AIM OF THE STUDY:To investigate the neuroprotective effect of aqueous extract of modified Wu-Zi-Yan-Zong granule (MWG), a traditional Chinese herbal medicine, against CoCl(2)-induced neurotoxicity in PC12 cells.
MATERIALS AND METHODS:Cell viability assay, apoptosis rate assay, ROS detection and mitochondrial membrane potential (MMP) assay were performed. In addition, cytochrome c, caspase-3, PARP and MAPKs were also detected by Western blotting.
RESULTS:MWG extract increased viability and suppresses early and middle/late stage apoptosis in a dose-dependent manner in CoCl(2)-induced PC12 cells. Moreover, MWG extract decreased the level of intracellular reactive oxygen species (ROS), increased MMP, regulated Bcl-2 family protein expression (Bcl-2 and Bcl-XL) and inhibited the release of cytochrome c from the mitochondria. In addition, MWG extract attenuated activation of caspase-3 and poly ADP-ribose polymerase (PARP) and inhibited the phosphorylation of ERK, c-Jun NH(2)-terminal kinase (JNK) and p38 MAPKs.
CONCLUSIONS:MWG extract exhibited significant neuroprotective effect on PC12 cells, and this effect may be associated with the suppression of ROS generation and inhibition of mitochondria-mediated caspase and MAPK signaling pathways.
巻・号 130(1)
ページ 13-8
公開日 2010-7-6
DOI 10.1016/j.jep.2010.03.018
PII S0378-8741(10)00180-7
PMID 20347949
MeSH Animals Apoptosis / drug effects Blotting, Western Cobalt / pharmacology* Drugs, Chinese Herbal* Membrane Potentials / drug effects Neuroprotective Agents / pharmacology* PC12 Cells Rats Reactive Oxygen Species / metabolism
IF 3.69
引用数 8
WOS 分野 PLANT SCIENCES PHARMACOLOGY & PHARMACY INTEGRATIVE & COMPLEMENTARY MEDICINE CHEMISTRY, MEDICINAL
リソース情報
ヒト・動物細胞 PC-12(RCB0009)