論文 - 詳細
| RRC ID | 19170 |
|---|---|
| 著者 | Eshraghi A, Maldonado-Arocho FJ, Gargi A, Cardwell MM, Prouty MG, Blanke SR, Bradley KA. |
| タイトル | Cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol. |
| ジャーナル | J Biol Chem |
| Abstract |
Cytolethal distending toxins (CDTs) are tripartite protein exotoxins produced by a diverse group of pathogenic Gram-negative bacteria. Based on their ability to induce DNA damage, cell cycle arrest, and apoptosis of cultured cells, CDTs are proposed to enhance virulence by blocking cellular division and/or directly killing epithelial and immune cells. Despite the widespread distribution of CDTs among several important human pathogens, our understanding of how these toxins interact with host cells is limited. Here we demonstrate that CDTs from Haemophilus ducreyi, Aggregatibacter actinomycetemcomitans, Escherichia coli, and Campylobacter jejuni differ in their abilities to intoxicate host cells with defined defects in host factors previously implicated in CDT binding, including glycoproteins, and glycosphingolipids. The absence of cell surface sialic acid sensitized cells to intoxication by three of the four CDTs tested. Surprisingly, fucosylated N-linked glycans and glycolipids, previously implicated in CDT-host interactions, were not required for intoxication by any of the CDTs tested. Finally, altering host-cellular cholesterol, also previously implicated in CDT binding, affected intoxication by only a subset of CDTs tested. The findings presented here provide insight into the molecular and cellular basis of CDT-host interactions. |
| 巻・号 | 285(24) |
| ページ | 18199-207 |
| 公開日 | 2010-6-11 |
| DOI | 10.1074/jbc.M110.112912 |
| PII | S0021-9258(19)57526-3 |
| PMID | 20385557 |
| PMC | PMC2881744 |
| MeSH | Animals Bacterial Toxins / chemistry* Bacterial Toxins / genetics* CHO Cells Campylobacter jejuni / metabolism Cholesterol / chemistry* Cholesterol / metabolism Cricetinae Cricetulus DNA Damage Escherichia coli / metabolism Glycolipids / chemistry Gram-Negative Bacteria / metabolism Haemophilus ducreyi / metabolism HeLa Cells Humans Mice NIH 3T3 Cells Polysaccharides / chemistry* Protein Binding |
| IF | 4.238 |
| 引用数 | 40 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LY-B(RCB1698) LY-B/cLCB1(RCB1699) |